Outer membrane vesicle (OMV) vaccine comprising
View Patent ↗A composition comprising (a) Neisseria meningitidis serogroup B outer membrane vesicles (OMVs), and (b) an immunogenic component selected from other Neisseria proteins, or immunogenic fragments thereof. Component (b) preferably includes a protein from a different NmB strain from that from which the OMV of component (a) is derived. The OMVs are preferably obtained by deoxycholate extraction. Optionally, the composition may also comprise a protective antigen against other pathogens.
1. A composition comprising a Neisseria meningitidis serogroup B outer membrane preparation mixed with a purified m741.pep protein comprising the amino acid sequence of SEQ ID NO: 22 or an immunogenic fragment of the m741.pep protein comprising ten or more consecutive amino acids of the amino acid sequence of SEQ ID NO: 22.
2. The composition of claim 1 , wherein the protein, the Neisseria meningitidis serogroup B outer membrane preparation, or both are adsorbed on Al(OH) 3 .
3. The composition of claim 1 , wherein the outer membrane preparation comprises outer membrane vesicles (OMVs) of the Neisseria meningitidis serogroup B.
4. The composition of claim 3 , wherein the OMVs are a deoxycholate extract from the Neisseria meningitidis serogroup B.
5. The composition of claim 2 , wherein the protein is adsorbed on Al(OH) 3 .
6. The composition of claim 1 , further comprising an isolated or purified Neisseria meningitidis protein selected from the group consisting of:
(a) protein ORF1 comprising the amino acid sequence of SEQ ID NO: 16;
(b) protein ORF40 comprising the amino acid sequence of SEQ ID NO: 17;
(c) protein ORF46 comprising the amino acid sequence of SEQ ID NO: 18;
(d) protein 287 comprising the amino acid sequence of SEQ ID NO: 19;
(e) protein 919 comprising the amino acid sequence of SEQ ID NO: 21 and
(f) transferrin binding protein B (TbpB) comprising the amino acid sequence of SEQ ID NO: 15.
7. The composition of claim 6 , comprising the protein ORF1 comprising the amino acid sequence of SEQ ID NO: 16.
8. The composition of claim 6 , comprising the protein ORF40 comprising the amino acid sequence of SEQ ID NO: 17.
9. The composition of claim 6 , comprising the protein ORF46 comprising the amino acid sequence of SEQ ID NO: 18.
10. The composition of claim 6 , comprising the protein 287 comprising the amino acid sequence of SEQ ID NO: 19.
11. The composition of claim 6 , comprising the protein 919 comprising the amino acid sequence of SEQ ID NO: 21.
12. The composition of claim 6 , comprising the TbpB comprising the amino acid sequence of SEQ ID NO: 15.
13. A composition comprising a Neisseria meningitidis serogroup B outer membrane preparation isolated from a recombinant serogroup B Neisseria meningitidis that hyperproduces a protein from a strain of Neisseria meningitidis serogroup B different from the recombinant serogroup B Neisseria meningitidis and wherein the protein is m741.pep comprising the amino acid sequence of SEQ ID NO: 22 or an immunogenic fragment of the m741.pep protein comprising ten or more consecutive amino acids of the amino acid sequence of SEQ ID NO: 22.
14. The composition of claim 13 , wherein the outer membrane preparation comprises outer membrane vesicles (OMVs) of the Neisseria meningitidis serogroup B.
15. The composition of claim 14 , wherein the OMVs are an isolated deoxycholate extract from the recombinant Neisseria meningitidis serogroup B.
16. The composition of claim 13 , wherein the Neisseria meningitidis serogroup B outer membrane preparation is adsorbed on Al(OH) 3 .
17. The composition of claim 13 , further comprising an isolated or purified Neisseria meningitidis protein selected from the group consisting of:
(a) protein ORF1 comprising the amino acid sequence of SEQ ID NO: 16;
(b) protein ORF40 comprising the amino acid sequence of SEQ ID NO: 17;
(c) protein ORF46 comprising the amino acid sequence of SEQ ID NO: 18;
(d) protein 287 comprising the amino acid sequence of SEQ ID NO: 19;
(e) protein 919 comprising the amino acid sequence of SEQ ID NO: 21 and
(f) transferrin binding protein B (TbpB) comprising the amino acid sequence of SEQ ID NO: 15.