Column packaging for chromatography
The present invention provides a packing for chromatography. Specifically, it provides a packing for chromatography in which a hydrophilic polymeric material is bound to the surface of a porous carrier, and a carbon chain is chemically bound inside the pore. By the use of the packing according to the present invention for analysis of low molecular weight compounds in a biological sample, a pretreatment such as removal of proteins etc. becomes unnecessary, and the biological sample can be directly analyzed.
1. A packing for chromatography in which a hydrophilic polymeric material is bound to a surface of a porous silica gel, and a carbon chain is chemically bound inside the pore of the silica gel, wherein said packing is prepared by a step of contacting the pore of the silica gel with a compound having said carbon chain.
2. The packing for chromatography according to claim 1 , in which the hydrophilic polymeric material has a molecular weight of from 1,000 to 2,000,000.
3. The packing for chromatography according to claim 1 , in which the hydrophilic polymeric material is methyl cellulose, ethyl cellulose, hydroxypropyl cellulose, polyvinylpyrrolidone or hexadimethrine bromide.
4. The packing for chromatography according to claim 1 , in which the carbon chain is a linear or branched carbon chain optionally modified with a cyano group, allyl group, aryl group, amino group, carboxylic acid group, sulfonic acid group or sulfuric acid group.
5. The packing for chromatography according to claim 4 , in which the number of the carbon atoms in the linear or branched carbon chain is from 1 to 30.
6. The packing for chromatography according to claim 1 , in which the hydrophilic polymeric material is a chemically modified hydrophilic polymeric material.
7. The packing for chromatography according to claim 6 , in which the chemical modification is glycolization, reduction or carboxylation to introduce two carboxyl groups.
8. The packing for chromatography according to claim 1 , in which the compound having said carbon chain is a C 4 -C 12 chlorosilane, an octadecyltrichlorosilane or a substance used for introducing an ion exchange group.
9. The packing for chromatography according to claim 8 , in which the carbon chain is a linear or branched carbon chain optionally modified with a cyano group, allyl group, aryl group, amino group, carboxylic acid group, sulfonic acid group or sulfuric acid group.
10. A column packed with the packing for chromatography according to claim 1 .
11. A packing for chromatography in which a hydrophilic polymeric material is bound to a surface of a porous silica gel, and a carbon chain is chemically bound inside the pore of the silica gel, wherein said packing is prepared with a compound having the carbon chain.
12. The packing for chromatography according to claim 11 , in which the hydrophilic polymeric material has a molecular weight of from 1,000 to 2,000,000.
13. The packing for chromatography according to claim 11 , in which the hydrophilic polymeric material is methyl cellulose, ethyl cellulose, hydroxypropyl cellulose, polyvinylpyrrolidone or hexadimethrine bromide.
14. The packing for chromatography according to claim 11 , in which the carbon chain is a linear or branched carbon chain optionally modified with a cyano group, allyl group, aryl group, amino group, carboxylic acid group, sulfonic acid group or sulfuric acid group.
15. The packing for chromatography according to claim 14 , in which the number of the carbon atoms in the linear or branched carbon chain is from 1 to 30.
16. The packing for chromatography according to claim 11 , in which the hydrophilic polymeric material is a chemically modified hydrophilic polymeric material.
17. The packing for chromatography according to claim 16 , in which the chemical modification is glycolization, reduction or carboxylation to introduce two carboxyl groups.
18. The packing for chromatography according to claim 11 , in which the compound having said carbon chain is a C 4 -C 12 chlorosilane, an octadecyltrichlorosilane or a substance used for introducing an ion exchange group.
19. The packing for chromatography according to claim 18 , in which the carbon chain is a linear or branched carbon chain optionally modified with a cyano group, allyl group, aryl group, amino group, carboxylic acid group, sulfonic acid group or sulfuric acid group.
20. A column packed with the packing for chromatography according to claim 11 .