IP Library › Granted Patent US 7,410,769
Granted Patent B2
US 7,410,769 · App. 10/182,303 · Granted Aug 12, 2008

Peptide biosensors for anthrax protease

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Quick Facts
Patent No.
US 7,410,769
App. No.
10/182,303
Granted
Aug 12, 2008
Kind
B2
Abstract

The present invention provides a protease biosensor that can be used to detect the presence of the lethal factor protease from Bacillus anthracis, as well as methods for using the protease biosensor.

Claims (14)

1. An isolated protease biosensor consisting of:

a) a peptide consisting of the sequence of SEQ ID NO: 1

b) a fluorescent donor molecule attached to the peptide that is capable of participating in fluorescence resonance energy transfer; and

c) an acceptor molecule attached to the peptide that has an absorption spectrum overlapping the emission spectrum of the donor molecule, wherein the fluorescence donor molecule and the acceptor molecule are attached to amino acid residues that are on opposite sides of a protease cleavage site in the peptide.

2. An isolated protease biosensor consisting of:

a) a peptide consisting of the sequence of SEQ ID NO: 2

b) a fluorescent donor molecule attached to the peptide that is capable of participating in fluorescence resonance energy transfer; and

c) an acceptor molecule attached to the peptide that has an absorption spectrum overlapping the emission spectrum of the donor molecule, wherein the fluorescence donor molecule and the acceptor molecule are attached to amino acid residues that are on opposite sides of a protease cleavage site in the peptide.

3. An isolated protease biosensor consisting of:

a) a peptide consisting of an amino acid sequence of the general formula II:

R1-R2-R3-R4-R5-Pro-R6-R7-R-8-R9

wherein R1 is Lys; R2 is Lys; R3 is Lys; R4 is Pro; R5 is Thr; R6 is Ile; R7 is Gln; R8 is Leu; and R9 is Asn;

b) a fluorescent donor molecule attached to the peptide that is capable of participating in fluorescence resonance energy transfer; and

c) an acceptor molecule attached to the peptide that has an absorption spectrum overlapping the emission spectrum of the donor molecule, wherein the fluorescence donor molecule and the acceptor molecule are attached to amino acid residues that are on opposite sides of a protease cleavage site in the peptide.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 7, 2005
From: CARL ZEISS JENA GMBH; CARL ZEISS MICROIMAGING, INC.
To: CELLOMICS, INC.
Reel/Frame 016864/0619 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 17, 2003
From: CELLOMICS, INC.
To: CARL ZEISS JENA GMBH
Reel/Frame 014692/0942 →