Genetic engineering of plants through manipulation of lignin biosynthesis
View Patent ↗The invention pertains to the genetically down regulating a lignin pathway p-coumarate Co-enzyme A ligase (CCL) in trees.
1. An isolated promoter comprising SEQ ID NO:6.
2. A polynucleotide construct comprising the promoter of claim 1 operably connected to a polynucleotide coding sequence.
3. The construct of claim 2 , wherein the coding sequence encodes a p-coumarate Co-enzyme A amino acid sequence.
4. A method of expressing a polynucleotide coding sequence in the epidermis of a plant, comprising:
(a) introducing the DNA construct of claim 2 into a plant cell; and
(b) regenerating a transgenic plant from the plant cell, wherein the polynucleotide coding sequence is expressed in the epidermis of the plant.
5. The method of claim 4 , wherein the polynucleotide coding sequence confers a trait selected from the group consisting of: altered flavanoid content, altered disease resistance, altered stress resistance, altered growth and combinations thereof, relative to a control plant.
6. A transgenic plant produced by the method of claim 4 .