Laser microscope
View Patent ↗A laser microscope includes a laser light emitting system which emits a pump beam and a Stokes beam having different frequencies, and a common optical fiber having one end and the other end. The pump beam and the Stokes beam emitted from the laser light emitting system are incident on the one end thereof and emitted from the other end thereof. A beam irradiating mechanism condenses and irradiates the pump beam and the Stokes beam emitted from the other end of the optical fiber, onto a sample via the objective lens, thereby making an anti-Stokes beam be emitted from the sample. A photo detector detects the anti-Stokes beam emitted from the sample. A beam selecting mechanism allows only the anti-Stokes beam of the pump beam, the Stokes beam and the anti-Stokes beam to be received on the photo detector.
1. A laser microscope comprising:
a laser light emitting system which emits a pump beam and a Stokes beam having different frequencies;
a common optical fiber having a first end on which the pump beam and the Stokes beam emitted from the laser light emitting system are incident and a second end from which the pump beam and the Stokes beam are emitted;
a beam irradiating mechanism which includes an objective lens and which condenses and irradiates the pump beam and the Stokes beam emitted from the second end of the optical fiber onto a sample via the objective lens, thereby causing an anti-Stokes beam to be emitted from the sample;
a photo detector which detects the anti-Stokes beam emitted from the sample; and
a beam selecting mechanism which prevents the pump beam and the Stokes beam from being received on the photo detector.
2. A laser microscope according to claim 1 , wherein the optical fiber comprises a single mode fiber.
3. A laser microscope according to claim 1 , wherein the beam irradiating mechanism compares a pupil modulating mechanism which blocks a part of at least one of the pump bemin and the Stokes beam.
4. A laser microscope according to claim 1 , further comprising a scanning mechanism by which the sample is relatively scanned by the pump beam and the Stokes beam.
5. A laser microscope according to claim 4 , wherein the scanning mechanism comprises a stage which holds the sample and is movable in at least one direction.
6. A laser microscope according to claim 4 , wherein the scanning mechanism is provided on an optical path of the pump beam and the Stokes beam.
7. A laser microscope according to claim 1 , wherein the laser light emitting system comprises:
one laser light source which emits a beam having a predetermined frequency and a predetermined wavelength;
a beam splitter which separates the beam emitted from the laser light source into two beams; and
two lasers which convext respective frequencies and wavelengths of the two beams Separated by the beam splitter to have different predetermined values, and which respectively emit the pump bean and the Stokes beam.
8. A laser microscope according to claim 7 , further comprising an optical delay device on an optical path of the Stokes beam.
9. A laser microscope according to claim 7 , wherein the optical fiber comprises a single mode fiber.
10. A laser microscope according to claim 7 , wherein the beam irradiating mechanism comprises a pupil modulating mechanism which blocks a part of at least one of the pump beam and the Stokes beam.
11. A laser microscope according to claim 1 , wherein the laser light emitting system comprises:
two lasers which respectively emit the pump beam and the Stokes beam; and
a dispersion compensating optical system provided in an optical path of each of the pump beam and the Stokes beam to compensate for dispersion of the pump beam and the Stokes beam.
12. A laser microscope according to claim 11 , wherein one of the dispersion compensating optical systems comprises at least one prism.
13. A laser microscope according to claim 11 , wherein one of the dispersion compensating optical systems comprises a high dispersion glass.
14. A laser microscope according to claim 1 , wherein the laser light emitting system comprises:
two lasers which respectively emit the pump beam and the Stokes beam; and
an optical delay device on an optical path of the Stokes beam.
15. A laser microscope according to claim 11 , further comprising an optical delay device on the optical path of the Stokes beam.
16. A laser microscope according to claim 11 , further comprising a scanning mechanism which causes the pump beam and the Stokes beam to be scanned on the sample, and which is provided on an optical path of the pump beam and the Stokes beam.
17. A laser microscope according to claim 16 , further comprising another scanning mechanism which holds the sample and which is movable in at least one direction to scan the sample with respect to the pump beam and the Stokes beam.
18. A laser microscope according to claim 11 , wherein the beam irradiating mechanism comprises a pupil modulating mechanism which blocks a part of at least one of the pump beam and the Stokes beam.