Rapidly cleavable sumo fusion protein expression system for difficult to express proteins
View Patent ↗A recombinant expression system for the expression of a poly amino acid, peptide or protein is provided. The poly amino acid of interest is expressed as a fusion protein that includes an amino acid sequence recognized and cleaved by a Ulp1 protease. The amino acid sequence joined to the poly amino acid of interest is preferably from a SUMO (small ubiquitin-like molecule) protein. This sequence imparts favorable solubility and refolding properties to the fusion protein. A purification tag may also be incorporated into the fusion protein for ease of isolation. The Ulp1 protease used to cleave the fusion protein may be the Ulp1 protease or the active Ulp1 protease fragment, Ulp1(403-621). The Ulp1 protease rapidly and specifically cleaves the fusion proteins of the invention at the Ulp1 cleavage site. The amino acid sequence recognized by a Ulp1 protease is cleaved asymetrically to leave only an N-terminal serine joined to the poly amino acid of interest. This recombinant expression system is particularly advantageous for expression and rapid and highly specific cleavage and purification of poly amino acids that have low solubilities or are difficult to express in other systems.
1. A method of expressing a recombinant poly-amino acid of interest, the method comprising:
i.) providing a vector encoding a fusion protein comprising the recombinant poly-amino acid of interest located C-terminally to a purification tag and wherein the purification tag and the recombinant poly-amino acid of interest are separated by an expression enhancing amino acid sequence cleavable by Ulp1(403-621) protease, wherein the poly-amino acid of interest is expressed at 1% or less than 1% of total protein by weight or is expressed defectively when it does not contain an expression enhancing amino acid sequence in a recombinant expression system; and
ii.) expressing the fusion protein from the vector in a suitable recombinant host cell.
2. A method for purifying a recombinant poly-amino acid of interest, the method comprising:
i.) providing a vector encoding a fusion protein comprising the recombinant poly-amino acid of interest located C-terminally to a purification tag and wherein the purification tag and the recombinant poly-amino acid of interest are separated by an expression enhancing amino acid sequence cleavable by Ulp1(403-621) protease, wherein the poly-amino acid of interest is expressed at 1% or less than 1% of total protein by weight or is expressed defectively when it does not contain an expression enhancing amino acid sequence in a recombinant expression system; and
ii.) expressing the fusion protein from the vector in a suitable recombinant host cell;
iii.) purifying the fusion protein by means of the purification tag; and
iv.) cleaving the fusion protein with a Ulp1 protease or an active fragment of a Ulp1 protease to release the poly-amino acid of interest; and
v.) purifying the recombinant poly-amino acid of interest.
3. The method of claim 2 , further comprising cleaving the fusion protein with Ulp1(403-621) protease to release the recombinant poly-amino acid of interest having an N-terminal serine.
4. The method of claim 3 , further comprising purifying the recombinant poly-amino acid of interest having an N-terminal serine.
5. The method of claim 4 wherein the recombinant poly-amino acid of interest having an N-terminal serine is expressed and purified on a commercial scale.
6. The method of claim 1 , wherein the recombinant poly-amino acid of interest is expressed on a commercial scale.
7. The method of claim 2 , wherein the recombinant poly-amino acid of interest is expressed and purified on a commercial scale.