Biochip deposition system and method
View Patent ↗A biochip deposition system and method. The system comprises a deposit mechanism for depositing organic material in spots on a substrate; and a viewing system for detecting the spots on the substrate during a deposit operation and before the spots have dried, for determining if the spots have been correctly deposited, and if not, for generating a suitable output signal. The viewing system includes an illumination device for illuminating deposited spots at a relatively low angle relative to the substrate.
1. A method of monitoring a biochip deposit process, the method comprising:
a) detecting spots of organic material as they are deposited on a substrate and before the spots have dried, the detecting step including illuminating the substrate at an angle which is near parallel to the plane of the substrate;
b) determining if the spots have been correctly deposited; and,
c) if not, generating a suitable output signal.
2. A method according to claim 1 , wherein the illumination angle is in the range 0-2° with respect to the plane of the substrate.
3. A method according to claim 2 , wherein the illumination angle is the range 0-1° with respect to the plane of the substrate.
4. A method according to claim 1 , wherein steps b) and c) are carried out after completion of the deposit process.
5. A method according to claim 1 , wherein step (a) includes obtaining an image of the spots using a camera while there is no relative movement between the camera and the substrate.
6. A method according to claim 1 , wherein the output signal is generated if a spot volume lies outside a predetermined value or range.
7. A method according to claim 1 , wherein the output signal is generated if the ratio between orthogonal axes of a spot lies outside a predetermined value or range.
8. A method according to claim 1 , wherein the output signal is generated if a spot is not detected at an expected location.
9. A method according to claim 1 , wherein the output signal is generated if a spot is offset by more than a predetermined amount from an expected location.
10. A method according to claim 8 , wherein the expected location is defined with respect to other spots on the substrate.
11. A method according to claim 1 , further comprising marking the or part of the substrate in response to the generation of the output signal.
12. A method according to claim 11 , wherein the substrate is marked with a material which shows a detectable color, light level or densitometric change such as a colored dye, ink or wax.
13. A method according to claim 1 , further comprising detecting an identifier on the substrate.
14. A method according to claim 13 , wherein the detecting method is performed using an optical character or bar code reader.
15. A method according to claim 1 , further comprising storing details of the detected spot locations.
16. A method according to claim 15 , further comprising detecting an identifier on the substrate wherein the spot location details are stored in conjunction with the substrate identifier.
17. A method according to claim 1 , wherein the detecting step comprises locating the spots; detecting a first pair of edges of the substrate; detecting the position of the located spots relative to the substrate edges; and determining if each spot is located at an expected position relative to the substrate edges.
18. A method according to claim 17 , wherein the two first edges are substantially orthogonal.
19. A method according to claim 17 , further comprising detecting the position of the spots relative to a second pair of substantially parallel substrate edges.
20. A method according to claim 19 , wherein one of the first and second edges is common.
21. A method according to claim 1 , wherein the organic material includes a fluorescent dye.
22. A method according to claim 21 , further comprising detecting the spots of organic material on the substrate utilizing the fluorescent dye.
23. A method according to claim 1 , wherein the substrate is opaque or translucent.