DNA polymerases having amino acid substitutions and homologs thereof
View Patent ↗Thermostable DNA polymerases both in native form and having single amino acid substitutions and optionally N-terminal deletions are disclosed. These polymerases exhibit a substantial improvement of DNA sequencing performance compared to Taq DNA polymerase. The instant DNA polymerases also possess improved salt tolerance.
1. A purified recombinant thermostable DNA polymerase comprising the amino acid sequence set forth in FIG. 2 .
2. A method for synthesizing a fluorescently labeled polynucleotide, comprising the step of mixing the DNA polymerase of claim 1 with a primed template.
3. The method of claim 2 , wherein the primed template is a primed template in a chain termination sequencing reaction.
4. The method according to claim 2 , wherein the primed template is a primed template in a polymerase chain reaction.
5. A kit for synthesizing fluorescently labeled polynucleotides comprising the polymerase of claim 1 and a fluorescently labeled nucleotide.