Method for synthesizing cDNA

1. A kit for cDNA synthesis, which contains a mixture of a DNA polymerase from a thermophilic bacterium of the genus Bacillus and another DNA polymerase having a 3′-5′ exonuclease activity, wherein said DNA polymerase from a thermophilic bacterium of the genus Bacillus has a reverse transcription activity and a 3′-5′ exonuclease activity.

2. The kit according to claim 1 , wherein the DNA polymerase from a thermophilic bacterium of the genus Bacillus is a DNA polymerase from Bacillus caldotenax or a DNA polymerase from Bacillus stearothermophilus.

3. The kit according to claim 1 , wherein the DNA polymerase having a 3′-5′ exonuclease activity is an α-type DNA polymerase from an archaebacterium.

4. A kit for amplifying a cDNA by conducting a gene amplification reaction using a cDNA, synthesized according to a method in which a reverse transcription reaction is conducted in the presence of a DNA polymerase from a thermophilic bacterium of the genus Bacillus and another DNA polymerase having a 3′-5′ exonuclease activity, as a template, wherein said kit contains a mixture of a DNA polymerase from a thermophilic bacterium of the genus Bacillus and another DNA polymerase having a 3′-5′ exonuclease activity as well as a reagent for the gene amplification reaction, wherein said DNA polymerase from a thermophilic bacterium of the genus Bacillus has a reverse transcription activity and a 3′-5′ exonuclease activity.

5. The kit according to claim 4 , wherein the gene amplification reaction is PCR.

6. The kit according to claim 5 , which contains a DNA polymerase for PCR as the reagent for the gene amplification reaction.

7. The kit according to claim 6 , wherein the DNA polymerase having a 3′-5′ exonuclease activity in the mixture serves as the DNA polymerase for the PCR.

8. The kit according to claim 7 , wherein the DNA polymerase having a 3′-5′ exonuclease activity is an α-type DNA polymerase from an archaebacterium.