Assays for monitoring cell motility in real-time
View Patent ↗The invention relates to devices, devices for arraying biomolecules, including cells, methods for arraying biomolecules, assays for monitoring cellular movement, and systems for monitoring cellular movement. The devices include a support; a first layer configured to be placed in fluid-tight contact with the support, the first layer having an upper surface and defining a pattern of micro-orifices, each micro-orifice of the pattern of micro-orifices having walls and defining a micro-region on the support when the first layer is placed in fluid-tight contact with the support such that the walls of said each micro-orifice and the micro-region on the support together define a micro-well; and a second layer configured to be placed in fluid-tight contact with the upper surface of the first layer, the second layer defining a pattern of macro-orifices, each macro-orifice of the pattern of macro-orifices having walls and defining a macro-region when the first layer is placed in fluid-tight contact with the support and the second layer is placed in fluid-tight contact with the first layer such that the walls of the macro-orifice and the macro-region together define a macro-well.
1. An assay for monitoring cell movement comprising the steps of:
positioning a first layer to be in fluid-tight contact with a support, the first layer having an upper surface and defining a pattern of micro-orifices, each micro-orifice of the pattern of micro-orifices having walls and defining a micro-region on the support when the first layer is placed in fluid-tight contact with the support such that the walls of said each micro-orifice and the micro-region on the support together define a micro-well;
positioning a second layer to be in fluid-tight contact with an upper surface of the first layer, the second layer defining a pattern of macro-orifices, each macro-orifice of the pattern of macro-orifices having walls and defining a macro-region when the first layer is placed in fluid-tight contact with the support and the second layer is placed in fluid-tight contact with the first layer such that the walls of the macro-orifice and the macro-region together define a macro-well; each macro-region encompassing at least one micro-region;
immobilizing at least one cell of a plurality of cells in each respective micro-region on the support so as to situate the at least one cell within a corresponding micro-well, the cells thereby being arrayed on the support in a pattern that corresponds to the pattern of the micro-orifices;
allowing the cells to grow to confluency within the micro-regions;
providing at least one of a plurality of test agents to at least one macro-well and allowing said test agent to contact confluent cells;
removing said first and second layer;
monitoring cells for movement or lack of movement away from said micro-regions; and
correlating cellular movement or lack of movement away from said micro-regions with effect of said test agent on cellular movement.
2. The assay of claim 1 , wherein each macro-region encompasses a plurality of micro-regions.
3. The assay of claim 1 , wherein the step of immobilizing at least one cell comprises immobilizing a single cell in each micro-region.
4. The assay of claim 1 , wherein the step of immobilizing at least one cell comprises immobilizing a different type of cell in each micro-region.
5. The assay of claim 1 , wherein the step of immobilizing at least one cell includes immobilizing at least two different types of cell in a single micro-region.
6. The assay of claim 1 wherein the step of providing at least one test agent of a plurality of test agents comprises providing a different test agent in each macro-well.
7. The assay of claim 1 , further comprising the step of applying a coating to an upper surface of the support before positioning said first layer.
8. The assay of claim 7 , wherein the step of applying a coating to an upper surface of the support before positioning said first layer includes applying to the upper surface of the support a coating that is made of a material selected from the group consisting of proteins, protein fragments, peptides, small molecules, lipid bilayers, metals, self-assembled monolayers, cells, extracellular matrix proteins, hydrogels, and matrigel.
9. An assay for monitoring cell movement comprising the steps of:
positioning a first layer to be in fluid-tight contact with a support, the first layer having an upper surface and defining a pattern of micro-orifices, each micro-orifice of the pattern of micro-orifices having walls and defining a micro-region on the support when the first layer is placed in fluid-tight contact with the support such that the walls of said each micro-orifice and the micro-region on the support together define a micro-well;
immobilizing at least one cell of a plurality of cells in each respective micro-region on the support so as to situate the at least one cell within a corresponding micro-well, the cells thereby being arrayed on the support in a pattern that corresponds to the pattern of the micro-orifices;
allowing the cells to grow to confluency within the micro-regions;
removing the first layer from the support after the step of immobilizing;
positioning a second layer to be in fluid-tight contact with the support, the second layer defining a pattern of macro-orifices, each macro-orifice of the pattern of macro-orifices having walls and defining a macro-region when the second layer is placed in fluid-tight contact with the support such that the walls of the macro-orifice and the macro-region together define a macro-well; each macro-region encompassing at least one micro-region;
providing at least one of a plurality of test agents to at least one macro-well and allowing said test agent to contact confluent cells;
removing said second layer;
monitoring cells for movement or lack of movement away from said micro-regions;
correlating cellular movement or lack of movement away from said micro-regions with effect of said test agent on cellular movement.
10. The assay of claim 9 , wherein each macro-region encompasses a plurality of micro-regions.
11. The assay of claim 9 , wherein the step of immobilizing at least one cell comprises immobilizing a single cell in each micro-region.
12. The assay of claim 9 , wherein the step of immobilizing at least one cell comprises immobilizing a different type of cell in each micro-region.
13. The assay of claim 9 , wherein the step of immobilizing at least one cell includes immobilizing at least two different types of cell in a single micro-region.
14. The assay of claim 1 wherein the step of providing at least one test agent of a plurality of test agents comprises providing a different test agent in each macro-well.
15. The assay of claim 9 , further comprising the step of applying a coating to an upper surface of the support before positioning said first layer.
16. The assay of claim 15 , wherein the step of applying a coating to an upper surface of the support before positioning said first layer includes applying to the upper surface of the support a coating that is made of a material selected from the group consisting of proteins, protein fragments, peptides, small molecules, lipid bilayers, metals, self-assembled monolayers, cells, extracellular matrix proteins, hydrogels, and matrigel.
17. An assay for monitoring cell movement comprising the steps of:
positioning a first layer to be in fluid-tight contact with a support, the first layer having an upper surface and defining a pattern of micro-orifices, each micro-orifice of the pattern of micro-orifices having walls and defining a micro-region on the support when the first layer is placed in fluid-tight contact with the support such that the walls of said each micro-orifice and the micro-region on the support together define a micro-well;
positioning a second layer to be in fluid-tight contact with the support, the second layer comprising a plurality of rings, the rings defining a pattern of respective macro-orifices, each ring having walls and defining a macro-region when the second layer is placed in fluid-tight contact with the support such that the walls of the ring and the macro-region together define a macro-well; each macro-region encompassing at least one micro-region;
immobilizing at least one cell of a plurality of cells in each respective micro-region on the support so as to situate the at least one cell within a corresponding micro-well, the cells thereby being arrayed on the support in a pattern that corresponds to the pattern of the micro-orifices
allowing the cells to grow to confluency within the micro-regions;
providing at least one of a plurality of test agents to at least one macro-well and allowing said test agent to contact confluent cells;
removing said first and second layer;
monitoring cells for movement or lack of movement away from said micro-regions; and
correlating cellular movement or lack of movement away from said micro-regions with effect of said test agent on cellular movement.
18. The assay of claim 17 , wherein each macro-region encompasses a plurality of micro-regions.
19. The assay of claim 17 , wherein the step of immobilizing at least one cell comprises immobilizing a single cell in each micro-region.
20. The assay of claim 17 , wherein the step of immobilizing at least one cell comprises immobilizing a different type of cell in each micro-region.
21. The assay of claim 17 , wherein the step of immobilizing at least one cell includes immobilizing at least two different types of cell in a single micro-region.
22. The assay of claim 17 wherein the step of providing at least one test agent of a plurality of test agents comprises providing a different test agent in each macro-well.
23. The assay of claim 17 , further comprising the step of applying a coating to an upper surface of the support before positioning said first layer.
24. The assay of claim 23 , wherein the step of applying a coating to an upper surface of the support before positioning said first layer includes applying to the upper surface of the support a coating that is made of a material selected from the group consisting of proteins, protein fragments, peptides, small molecules, lipid bilayers, metals, self-assembled monolayers, cells, extracellular matrix proteins, hydrogels, and matrigel.
25. An assay for monitoring cell movement comprising the steps of:
positioning a layer to be in fluid-tight contact with the support, the layer defining a pattern of macro-orifices, each macro-orifice of the pattern of macro-orifices having walls and defining a macro-region when the layer is placed in fluid-tight contact with the support such that the walls of the macro-orifice and the macro-region together define a macro-well;
inserting each plug of a set of plugs in a respective macro-well, each of the plugs comprising a lower membrane placed in fluid-tight contact with the support when the layer is placed in fluid-tight contact with the support and the plug is received in a corresponding macro-well defined by the layer and the support, the lower membrane further defining a pattern of micro-orifices, wherein each micro-orifice has walls and defines a micro-region on the support when the plug is in fluid-tight contact with the support such that the walls of the micro-orifice and the micro-region together define a micro-well;
immobilizing a cell in at least one micro-region on the support so as to be situated within the micro-well, such that the cell is arrayed on the support in a pattern that corresponds to the first pattern of micro-orifices;
allowing the cells to grow to confluency within the micro-regions;
providing at least one of a plurality of test agents to at least one macro-well and allowing said test agent to contact confluent cells;
removing said layer containing said plugs;
monitoring cells for movement or lack of movement away from said micro-regions; and
correlating cellular movement or lack of movement away from said micro-regions with effect of said test agent on cellular movement.
26. The assay of claim 25 , wherein the step of immobilizing a cell comprises immobilizing a different type of cell in each micro-region.
27. The assay of claim 25 , wherein the step of immobilizing a cell includes immobilizing at least two different types of cells in a single micro-region.
28. The assay of claim 25 , wherein the step of providing at least one test agent of a plurality of test agents comprises providing a different test agent in each macro-well.
29. The assay of claim 25 , further comprising the step of applying a coating to an upper surface of the support before positioning said first layer.
30. The assay of claim 29 , wherein the step of applying a coating to an upper surface of the support before positioning said first layer includes applying to the upper surface of the support a coating that is made of a material selected from the group consisting of proteins, protein fragments, peptides, small molecules, lipid bilayers, metals, self-assembled monolayers, cells, extracellular matrix proteins, hydrogels, and matrigel.