Methods of assessing HIV integrase inhibitor therapy
View Patent ↗Methods and products for the evaluation of HIV treatment are provided. The methods are based on evaluating molecular events at the HIV integrase resulting in altered therapeutic efficacy of tho investigated compounds. The methods rely on providing an integrase gene and evaluating either through integrase gene genotyping or phenotyping.
1. A method for determining the susceptibility of at least one HIV to at least one drug, comprising:
i) obtaining at least one sample comprising HIV RNA from a patient, wherein the sample comprises at least one IN gene or a portion thereof;
ii) reverse-transcribing and amplifying said HIV RNA with primers specific for the IN region of the HIV genome to obtain an amplicon comprising the IN gene or a portion thereof, wherein at least one primer is selected from SEQ ID NO: 1-12 and 17-22;
iii) determining the nucleotide sequence of the amplicon or a portion thereof, and
iv) comparing the nucleotide sequence of the amplicon to the sequence of known HIV sequences to estimate the susceptibility of at least one HIV to as least one drug.
2. A method for determining the susceptibility of at least one HIV to at least one drug, comprising:
i) obtaining at least one sample comprising HIV DNA from a patient, wherein the sample comprise at least one IN gene or a portion thereof, wherein at least one primer is selected from SEQ ID NO: 1-12 and 17-22;
ii) amplifying said HIV DNA with primers specific for the IN region of the HIV genome to obtain an amplicon comprising the IN gene or a portion thereof;
iii) determining the nucleotide sequence of the amplicon or a portion thereof, and
iv) comparing the nucleotide sequence of the amplicon to the sequence of know HIV sequences to estimate the susceptibility of at least one HIV to at least one drug.
3. A kit useful for determining the susceptibility of at least one HIV to at least one drug according to any claim 1 or 2 , comprising at least one primer selected from SEQ ID NO: 1-12 and 17-22.
4. A primer as selected from SEQ ID NO 1-14.
5. A primer selected from SEQ ID NO 1-23.
6. A primer selected from SEQ ID NO 1-7 and SEQ ID NO 11-22.