Instrument for monitoring polymerase chain reaction of DNA
View Patent ↗An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.
1. An instrument comprising:
a plurality of spaced-apart reaction ingredient containers;
a light source adapted to direct an excitation beam toward the plurality of containers;
a Fresnel lens disposed along an excitation beam path, between the light source and the plurality of containers;
a detector disposed along an emission beam path and arranged to receive emission beams from the Fresnel lens; and
reaction ingredients disposed in each of the plurality of containers;
wherein the Fresnel lens is also disposed along an emission beam path between the plurality of containers and the detector, the Fresnel lens is adapted to direct emission beams from two or more of the plurality of containers to the detector, and the reaction ingredients include components for polymerase chain reaction.
2. An instrument comprising:
a plurality of spaced-apart reaction ingredient containers;
a light source adapted to direct an excitation beam toward the plurality of containers;
a Fresnel lens disposed along an excitation beam path, between the light source and the plurality of containers; and
a detector disposed along an emission beam path and arranged to receive emission beams from the Fresnel lens;
wherein the Fresnel lens is also disposed along an emission beam path between the plurality of containers and the detector, the Fresnel lens is adapted to direct emission beams from two or more of the plurality of containers to the detector, and the Fresnel lens comprises two or more lenses.
3. An instrument comprising:
a plurality of spaced-apart reaction ingredient containers;
a light source adapted to direct an excitation beam toward the plurality of containers;
a Fresnel lens disposed along an excitation beam path, between the light source and the plurality of containers;
a detector disposed along an emission beam path and arranged to receive emission beams from the Fresnel lens; and
reaction ingredients disposed in each of the plurality of containers;
wherein the Fresnel lens is also disposed along an emission beam path between the plurality of containers and the detector, the Fresnel lens is adapted to direct emission beams from more than one of the plurality of containers simultaneously, and the reaction ingredients include components for polymerase chain reaction.
4. An instrument comprising:
a plurality of spaced-apart reaction ingredient containers;
a light source adapted to direct an excitation beam toward the plurality of containers;
a Fresnel lens disposed along an excitation beam path, between the light source and the plurality of containers; and
a detector disposed along an emission beam path and arranged to receive emission beams from the Fresnel lens;
wherein the Fresnel lens is also disposed along an emission beam path between the plurality of containers and the detector, the Fresnel lens is adapted to direct emission beams from more than one of the plurality of containers simultaneously, and the Fresnel lens comprises two or more lenses.