IP Library › Granted Patent US 6,955,911
Granted Patent B2
US 6,955,911 · App. 10/221,200 · Granted Oct 18, 2005

Method of culturing microorganism

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Quick Facts
Patent No.
US 6,955,911
App. No.
10/221,200
Granted
Oct 18, 2005
Kind
B2
Abstract

In cultivating a microorganism having nitrile hydratase production ability, by allowing at least one of a ketose, such as fructose, and a sugar alcohol, such as mannitol, to be present, growth inhibition by cobalt ion is prevented, thereby achieving cultivation at a high concentration and with a high activity.

Claims (7)

1. A method of cultivating a microorganism having nitrile hydratase production ability in a medium substrate containing a sugar alcohol and/or ketose, and a cobalt ion.

2. The method according to claim 1 , wherein the sugar alcohol is represented by the general formula (I):

wherein n represents an integer from 2 to 4.

3. The method according to claim 1 , wherein the ketose is represented by the general formula (II):

wherein n represents 3.

4. The method according to claim 1 , wherein the concentration of cobalt is 15 mg/L or more in terms of CoCl 2 .

5. The method according to claim 1 , wherein the concentration of cobalt ion is 20 mg/L or more in terms of CoCl 3 .

Assignments (2)
CHANGE OF NAME Recorded Sep 5, 2017
From: MITSUBISHI RAYON CO., LTD.
To: MITSUBISHI CHEMICAL CORPORATION
Reel/Frame 043750/0834 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 12, 2002
From: RYUNO, KOICHIRO; KOBAYASHI, ETSUKO
To: MITSUBISHI RAYON CO., LTD.
Reel/Frame 013483/0117 →