Protease obtained from
The present invention relates to a Vibrio metscnikovii variants, more particularly to a biochemical characteristics of protease which is obtained from Vibrio metscnikovii variant strains such as Vibrio metschnikovii KS1, Vibrio metschnikovii KS1 transformed with pDSBCm, Vibrio metschnikovii KS1 transformed with pSBCm and Vibrio metschnikovii RH530 N-4-8 strain, the mother strain of Vibrio metschnikovii and to a detergent composition including the protease.
1. A protease obtained from Vibrio metschnikovii RH variant having a molecular weight in the range of 10,000–300,000, utilizing azocasein as a substrate, and having 10–12 pH at 40° C.–55° C.
2. The protease according to claim 1 , wherein Vibrio metschnikovii RH variant strains is obtained from more than one strains selected from the group consisting of Vibrio metschnikovii KS1 , Vibrio metschnikovii KS1 transformed with pDSBCm, Vibrio metschnikovii KS1 transformed with pSBCm and the mother strain, Vibrio metschinikovii RH530 N-4-8.
3. A detergent composition prepared by adding a stabilizer or a surfactant in an acceptable amount for a detergent to said protease (Vepe) of claim 1 .
4. The detergent composition according to claim 3 , wherein said surfactant is more than one selected from the group consisting of linear alkylbenzene sulfonate, .alpha.-olefin sulfonate sodium salt (AQS) and sodium lauryl ether sulfate.
5. The detergent composition according to claim 3 , wherein said stabilizer is selected from the group consisting of glycerol, ethylene glycol, calcium chloride dihydrate, sodium borate and sodium formate.