Method for producing recombinant adenovirus

1. A single chromatography step method for purifying adenovirus from a biological medium, the method comprising:

obtaining a biological medium comprising adenovirus;

performing a single chromatography step comprising anion-exchange chromatography on the biological medium; and

recovering the adenovirus with an adenoviral particle yield of at least 70% of the viral particles present in the biological medium prior to chromatography.

2. The method according to claim 1 , wherein the adenovirus is recombinant.

3. The method according to claim 2 , wherein the biological medium is a supernatant of encapsidation cells producing the adenovirus.

4. The method according to claim 2 , wherein the biological medium is a lysate of encapsidation cells producing the adenovirus.

5. The method according to claim 2 , wherein the biological medium is a prepurified solution of the adenovirus.

6. The method according to claim 2 , wherein the anion-exchange chromatography is on a support functionalized with a quaternary amine.

7. The method according to claim 6 , wherein the support is agarose, dextran, acrylamide, silica, poly(styrene-divinylbenzene), or ethylene glycol-methacrylate copolymer, or a mixture thereof.

8. The method of claim 6 , wherein the anion-exchange chromatography is performed on a Source Q, Mono Q, Q Sepharose, Poros HQ, Poros QE, Fractogel TMAE or Toyopearly Super Q.

9. The method of claim 8 , wherein the Source Q is Source 15Q.

10. The method according to claim 2 , further comprising performing ultrafiltration on the biological medium, or the anion-exchange chromatography fraction, or both.

11. The method of claim 10 , wherein the ultrafiltration is tangential ultrafiltration on a membrane having a cut-off ranging from 300 to 500 kDa.