Methods and compositions for categorizing patients
View Patent ↗The disclosure provides, among other things, molecular markers for categorizing the neoplastic state of a patient, methods for using the molecular markers in diagnostic tests, nucleic acid and amino acid sequences related to the molecular markers, reagents for detection of molecular markers, and methods for identifying candidate molecular markers in highly parallel gene expression data.
1. An antibody that binds specifically to a polypeptide consisting of the amino acid sequence of SEQ ID NO:3.
2. The antibody of claim 1 , wherein the antibody binds the polypeptide with a dissociation constant of less than 10 −6 M.
3. The antibody of claim 1 , wherein the antibody is a monoclonal antibody.
4. The antibody of claim 1 , wherein the antibody is effective for binding specifically to the polypeptide in a blood sample.
5. The antibody of claim 1 , wherein the antibody is effective for binding specifically to the polypeptide in a sample comprising cells from a coon neoplasia.
6. A method for producing a monoclonal antibody of claim 3 , the method comprising:
(a) culturing a hybridoma cell culture that produces a monoclonal antibody that binds specifically to the polypeptide; and
(b) obtaining the monoclonal antibody from the cell culture.
7. A method for generating a monoclonal antibody of claim 3 , the method comprising:
(a) administering to a mouse an amount of an immunogenic composition comprising a polypeptide consisting of amino acid sequence of SEQ ID NO:3 effective to stimulate a detectable immune response;
(b) obtaining antibody-producing cells from the mouse and fusing the antibody-producing cells with myeloma cells to obtain antibody-producing hybridomas;
(c) culturing a hybridoma cell culture that produces the monoclonal antibody; and
(d) obtaining the monoclonal antibody from the cell culture.
8. The method of claim 7 , wherein the antibody-producing hybridomas are tested to determine whether the antibody-producing hybridomas produce an antibody that binds the polypeptide in an assay selected from the group consisting of: an enzyme-linked immunoabsorbent assay, a surface plasmon resonance (SPR) binding assay, a western blot, immunohistochemistry, fluorescence activated cell sorting and immunoprecipitation assay.