Method for promoting neovascularization
View Patent ↗The present invention relates to a method for enhancing angiogenic activity to promote neovascularization comprising administering to a subject a formulation comprising a synergistically effective amount of a TWEAK agonist and an angiogenic factor.
1. A method for enhancing endothelial cell proliferation in an in vitro culture comprising the step of adding to said culture, a synergistically effective amount of TWEAK and basic fibroblast growth factor (bFGF).
2. A method for enhancing angiogenic activity in a mammal to promote neovascularization comprising the step of administering to said mammal a synergistically effective amount of TWEAK and basic fibroblast growth factor (bFGF).
3. The method according to claim 2 , wherein said method is used in the treatment of a myocardial ischemic condition in said mammal.
4. The method according to claim 2 , wherein said method is used to promote wound healing in said mammal.
5. The method according to claim 1 or 2 , wherein said TWEAK is a soluble recombinant TWEAK protein.
6. The method according to claim 1 or 2 , wherein said synergistically effective amount comprises basic fibroblast growth factor (bFGF) and TWEAK in a weight per weight ratio of at least about 1:100 to about 1:200.
7. The method according to claim 2 , wherein said method is used to promote neovascularization of transplanted or grafted tissues or organs in said mammal.
8. The method according to any one of claims 2 , 3 , 4 or 7 , wherein said mammal is a human.
9. The method according to any one of claims 2 , 3 , 4 or 7 , wherein said TWEAK and said basic fibroblast growth factor (bFGF) are administered by a route selected from the group consisting of: local injection, topical administration, parenteral administration, intranasal administration, intratracheal administration, pulmonary administration, oral administration, intravenous administration, intramuscular administration and subcutaneous administration.