Endoglucanase enzyme NCE5 and cellulase preparations containing the same
View Patent ↗There is provided an endoglucanase enzyme, which is useful for reducing fuzz of regenerated cellulose-containing fabrics, improving the touch and appearance, color clarification, localized variation in color, reducing stiffness and using it as components of a detergent, as well as deinking waste paper and improving freeness of paper pulp. A cDNA coding for the endoglucanase enzyme NCE5 was cloned and its DNA sequence and amino acid sequence derived from it were determined.
1. An isolated protein having endoglucanase activity, which comprises positions 19 to 223 of SEQ ID NO:1.
2. The protein of claim 1 , further comprising positions 1 to 18 of SEQ ID NO:1 in the N-terminal side.
3. The protein according to claim 1 , wherein it is isolated from a mold.
4. The protein according to claim 3 , wherein the mold belongs to the genus Humicola.
5. The protein according to claim 4 , wherein the mold belongs to Humicola insolens.
6. An isolated polynucleotide component which comprises a polynucleotide sequence coding for the protein of claim 1 .
7. An isolated polynucleotide component which comprises the polynucleotide sequence described in SEQ ID NO:2.
8. An expression vector which comprises the polynucleotide sequence described in claim 6 .
9. An isolated host cell transformed with the expression vector described in claim 8 .
10. The host cell according to claim 9 , wherein it is a yeast or a mold.
11. The host cell according to claim 10 , wherein the yeast belongs to the genus Saccharomyces , the genus Hansenula or the genus Pichia.
12. The host cell according to claim 11 , wherein the yeast is Saccharomyces cerevisiae.
13. The host cell according to claim 10 , wherein the mold belongs to the genus Humicola , the genus Aspergillus , the genus Trichoderma , the genus Fusarium or the genus Acremonium.
14. The host cell according to claim 10 , wherein the mold is Humicola insolens, Aspergillus niger or Aspergillus oryzae, Trichoderma viride, Fusarium oxysporum or Acremonium cellulolyticus.
15. A method for producing an isolated protein having endoglucanase activity, which comprises positions 19 to 223 of SEQ ID NO:1, which comprises the steps of culturing the host cell of claim 9 and subsequently collecting the protein from the host cell and/or its culture mixture.
16. An isolated protein having endoglucanase activity, which comprises positions 19 to 223 of SEQ ID NO:1 produced by culturing a host cell transformed with the expression vector of claim 8 and collecting the protein from the host cell and/or its culture mixture.
17. A cellulase preparation which comprises the protein of claim 1 .
18. A method for treating a cellulose-containing fabric, which comprises a step of allowing the cellulose-containing fabric to contact with the protein of claim 1 .
19. A method of reducing the rate at which a cellulose-containing fabric becomes fuzzy or of reducing fuzzing in a cellulose-containing fabric, which comprises a step of allowing the cellulose-containing fabric to contact with the protein of claim 1 .
20. A method of weight loss treatment for a cellulose-containing fabric to improve its touch and appearance, which comprises a step of allowing the cellulose-containing fabric to contact with the protein of claim 1 .
21. A method of providing color clarification of colored cellulose-containing fabric, which comprises a step of treating the colored cellulose-containing fabric with the protein of claim 1 .
22. A method for providing a localized variation in color of a colored cellulose-containing fabric, which comprises a step of treating the colored cellulose-containing fabric with the protein of claim 1 .
23. A method of reducing the rate at which a cellulose-containing fabric becomes stiff or of reducing stiffness in a cellulose-containing fabric, which comprises a step of treating the cellulose-containing fabric with the protein of claim 1 .
24. The method according to claim 18 , wherein the treatment of fabric is carried out through soaking, washing and rinsing of the fabric.
25. A detergent additive agent which comprises the protein of claim 1 in a non-scattering granular form or a stabilized liquid form.
26. A detergent composition which comprises the protein of claim 1 .
27. A method for deinking waste paper, which comprises treating the waste paper with the protein of claim 1 and a deinking agent.
28. A method for improving freeness of paper pulp, which comprises a step of treating paper pulp with the protein of claim 1 .
29. A method for improving digestion ability of animal feed, which comprises a step of treating a cellulose-containing fiber with the protein of claim 1 .