Cartridge for automatic measurement and measuring method using it
View Patent ↗A cartridge for use in measuring a component to be measured contained in a sample includes a diluting well for diluting a predetermined amount of the sample to a desired dilution, and a reaction well in which the component to be measured contained in the sample and a substance specifically reacting therewith are allowed to react. A diluting solution is filled in the diluting well in a predetermined amount to provide the desired dilution based on a type of the component to be measured when the predetermined amount of the sample is dispensed in the diluting well by a uniform operation.
1. A measuring method for a component to be measured contained in a sample, comprising:
providing a cartridge comprising at least two or more lines of well groups arranged in parallel or providing plural cartridges each having at least one line of well groups, wherein each well group comprises a diluting well for diluting a predetermined amount of the sample to a desired dilution, and a reaction well in which the component to be measured contained in the sample and a substance specifically reacting therewith are allowed to react, wherein a diluting solution is filled in the diluting well of each well group in a predetermined amount to provide the desired dilution depending on a type of the component to be measured and then sealing the cartridge or plural cartridges using a cartridge seal or cartridge seals;
piercing the cartridge seal or cartridge seals and dispensing the predetermined amount of sample in the diluting well of each well group already filled with the predetermined amount of diluting solution with a dispensing mechanism comprising multiple dispensing elements in a uniform operation to thereby dilute the sample to the desired dilution in each diluting well of each well group;
reacting the component to be measured in the diluted sample with the substance specifically reacting therewith; and
measuring an amount of a reaction product.
2. A measuring method according to claim 1 , wherein a plurality of kinds of different components to be measured are concurrently measured with regard to the two or more lines of well groups provided in the cartridge, or with regard to the at least one line of well groups provided by the plurality of cartridges.
3. A measuring method according to claim 1 , wherein the plurality of cartridges are provided with at least one cartridge of the plurality of cartridges having at least one well filled with at least one of a reagent and a solution used in the measurement of the component to be measured contained in the sample.
4. A measuring method according to claim 1 , wherein the reaction between the component to be measured and the substance specifically reacting therewith is an immunological reaction.
5. A measuring method according to claim 4 , wherein the immunological reaction is a reaction in which the component to be measured in the sample and the substance immunologically specifically reacting therewith are allowed to react to form a first immune complex, and the first immune complex and a label immunologically specifically reacting therewith are allowed to react to form a second immune complex, and wherein the amount of the label in the second immune complex formed by the reaction is measured.
6. A measuring method according to claim 1 , wherein the cartridge comprising the two or more lines of well groups arranged in parallel is provided.
7. A measuring method according to claim 1 , wherein each well group further comprises at least one of a reagent-containing well for containing a reagent used in the measurement, a sample-dispensing well for dispensing the sample, a washing well for performing washing of a reaction product, and a measuring well for performing measurement of the reaction product.
8. A measuring method according to claim 1 , wherein plural cartridges each having at least one line of well groups are provided.