Human coagulation factor VII polypeptides
The present invention relates to novel human coagulation Factor VIIa variants having coagulant activity as well as polynucleotide constructs encoding such variants, vectors and host cells comprising and expressing the polynucleotide, pharmaceutical compositions, uses and methods of treatment.
1. A Factor VII polypeptide comprising at least one amino acid substitution relative to the amino acid sequence of SEQ ID NO:1, wherein said substitution is replacement with any other amino acid of one or more amino acids selected from the group consisting of amino acids, 313, 314, 317-329, 364, 366, 373, and 376 of SEQ ID NO:1, wherein the ratio between the activity of said Factor VII polypeptide and the activity of the native Factor VIIa polypeptide having a sequence as shown in SEQ ID NO:1 is at least about 1.25, when the activity is measured in an in vitro hydrolysis assay.
2. A Factor VII polypeptide comprising at least one amino acid substitution relative o the amino acid sequence of SEQ ID NO:1, wherein said substitution is replacement with any other amino acid of one or more amino acids selected from the group consisting of amino acids 313, 314, and 317-329 of SEQ ID NO:1.
3. The Factor VII polypeptide according to claim 1 , wherein S314 is replaced with any other ammo acid.
4. The Factor VII polypeptide according to claim 1 , further comprising an additional replacement with any other amino acid of at least one amino acid in the remaining positions in the protease domain.
5. The Factor VII polypeptide according to claim 4 , wherein at the most 20 additional ammo acids in the remaining positions in the protease domain have been replaced with any other amino acids.
6. The Factor VII polypeptide according to claim 4 , wherein at least one amino acid corresponding to an amino acid at a position selected from 330-339 of SEQ ID NO:1 has been replaced with any other amino acid.
7. The Factor VII polypeptide according to claim 4 , wherein the additional replacement comprises replacement of one or more amino acids selected from the group consisting of amino acids L305, K157, K337, D334, S336, V158, E296, and M298 of SEQ ID NO:1.
8. A Factor VII polypeptide according to claim 1 having one amino acid substitution relative to the amino acid sequence of SEQ ID NO: 1, wherein said substitution is of an amino acid selected from the group consisting of amino acids 313, 314, and 317-329, 364, 366, 373, and 376 of SEQ ID NO:1.
9. A Factor VII polypeptide according to claim 1 having two amino acid substitutions relative to the amino acid sequence of SEQ ID NO:1, wherein said substitutions are of amino acids selected from the group consisting of amino acids 313-329, 364, 366, 373, and 376 of SEQ ID NO:1.
10. A Factor VII polypeptide according to claim 1 having three amino acid substitutions relative to the amino acid sequence of SEQ ID NO:1, wherein said substitutions are of amino acids selected from the group consisting of amino acids 313-329, 364, 366, 373, and 376 of SEQ ID NO:1.
11. A Factor VII polypeptide having four amino acid substitution relative to the amino acid sequence of SEQ ID NO:1, wherein said substitutions are of amino acids selected from the group consisting of amino acids 313-329, 364, 366, 373, and 376 of SEQ ID NO:1.
12. The Factor VII polypeptide according to claim 1 , wherein S314 has been replaced by an amino acid selected from the group consisting of Gly and Glu.
13. The Factor VII polypeptide according to claim 1 , wherein said S314 has been replaced by Glu.
14. The Factor VII polypeptide according to claim 13 , further comprising replacement of K316 by Gln.
15. The Factor VII polypeptide according to claim 1 , wherein the amino acid has been replaced with any other amino acid that can be encoded by a polynucleotide construct.
16. The Factor VII polypeptide according to claim 1 , wherein said Factor VII polypeptide is human Factor VII.
17. The Factor VII polypeptide according to claim 1 , wherein said Factor VII polypeptide is human Factor VIIa.
18. The Factor VII polypeptide according to claim 1 , wherein said ratio is at least about 2.
19. The Factor VII polypeptide according to claim 9 , wherein said polypeptide is selected from the group consisting of S314E/K316H-FVII and S314E/K316Q-FVII.
20. A pharmaceutical composition comprising
(i) a Factor VII polypeptide, wherein said polypeptide comprises at least one amino acid substitution relative to the amino acid sequence of SEQ ID NO:1 and wherein said substitution is replacement with any other amino acid of one or more amino acids selected from the group consisting of amino acids 313, 314, and 317-329, 364, 366, 373, and 376 of SEQ ID NO:1; and,
(ii) a pharmaceutically acceptable carrier.
21. A pharmaceutical composition comprising (i) a Factor VII polypeptide as defined in claim 1 and, (ii) a pharmaceutically acceptable carrier.
22. A method for the treatment of bleeding disorders or bleeding episodes or for the enhancement of the normal haemostatic system in a subject, the method comprising administering to said subject an effective amount for said treatment or enhancement of a Factor VII polypeptide comprising at least one amino acid substitution relative to the amino acid sequence of SEQ ID NO:1, wherein said substitution is replacement with any other amino acid of one or more amino acids selected from the group consisting of amino acids 313-329, 364, 366, 373, and 376 of SEQ ID NO:1, wherein the ratio between the activity of said Factor VII polypeptide and the activity of the native Factor VIIa polypeptide having a sequence as shown in SEQ ID NO: 1 is at least about 1.25, when the activity is measured in an in vitro hydrolysis assay.