Detection and typing of human papillomavirus using PNA probes
View Patent ↗The invention provides materials and methods for detection and typing of HPV infection using PNA probes. More specifically, methods are provided for detecting high-risk types of HPV infection with minimal numbers of PNA probes or using PNA probes to selectively amplify only high-risk types of HPV. Novel primer sequences are also provided.
1. An essentially pure nucleic acid comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO: 9, 10, 12, 13, 17-19 and 21.
2. The nucleic acid of claim 1 , comprising SEQ ID NOS: 9.
3. The nucleic acid of claim 1 , comprising SEQ ID NOS: 10.
4. The nucleic acid of claim 1 , comprising SEQ ID NOS: 12.
5. The nucleic acid of claim 1 , comprising SEQ ID NOS: 13.
6. The nucleic acid of claim 1 , comprising SEQ ID NOS: 17.
7. The nucleic acid of claim 1 , comprising SEQ ID NOS: 18.
8. The nucleic acid of claim 1 , comprising SEQ ID NOS: 19.
9. The nucleic acid of claim 1 , comprising SEQ ID NOS: 21.
10. A method for amplifying a target nucleic acid comprising selecting a primer selected from the group consisting of SEQ ID NOS: 9, 10, 12, 13 17-19 and 21, and amplifying said target nucleic acid.
11. The method of claim 10 , wherein the method comprises a polymerase chain reaction.
12. The method of claim 10 , further comprising selecting a primer selected from the group consisting of SEQ ID NOS: 8 and 16.
13. The method of claim 10 , wherein a target nucleic acid of the amplification is a high risk strain of human papilloma virus.
14. The method of claim 10 , further comprising selecting a probe comprising a nucleic acid analog to block full length amplification of a non-target nucleic acid.
15. The method of claim 13 , wherein the non-target nucleic acid is of a low risk strain of human papilloma virus.