Compositions isolated from forage grasses and methods for their use
View Patent ↗Isolated polynucleotides encoding polypeptides active in lignin, fructan and tannin biosynthetic pathways are provided, together with expression vectors and host cells comprising such isolated polynucleotides. Methods for the use of such polynucleotides and polypeptides are also provided.
1. An isolated polynucleotide comprising SEQ ID NO: 151.
2. A genetic construct comprising the isolated polynucleotide of claim 1 .
3. An isolated transgenic cell comprising the construct according to claim 2 .
4. A recombinant construct comprising, in the 5′–3′ direction:
(a) a gene promoter sequence;
(b) a polynucleotide sequence comprising nucleotides 87–1,718 of SEQ ID NO: 151; and
(c) a gene termination sequence.
5. The construct of claim 4 , wherein the polynucleotide is in the sense orientation.
6. A transgenic plant cell comprising the construct of claim 4 .
7. A transgenic plant comprising the transgenic plant cell according to claim 6 , or fruit or seeds or progeny thereof, wherein the fruit, seeds or progeny thereof comprise a recombinant construct comprising, in the 5′–3′ direction:
(a) a gene promoter sequence;
(b) a polynucleotide sequence comprising nucleotides 87–1,718 of SEQ ID NO: 151; and
(c) a gene termination sequence.
8. A method for modulating the lignin composition of a plant, comprising stably incorporating into the genome of the plant the polynucleotide of claim 1 , expressing said polynucleotide, thereby modulating the lignin composition of the plant.
9. The method of claim 8 , wherein the plant is a grass.
10. The method of claim 9 , wherein the plant is selected from the group consisting of Lolium perenne and Festuea arundinacea .
11. The method of claim 8 comprising stably incorporating into the genome of the plant a recombinant construct comprising, in the 5′–3′ direction:
(a) a gene promoter sequence;
(b) a polynucleotide sequence comprising nucleotides 87–1,718 of SEQ ID NO:151; and
(c) a gene termination sequence.
12. A method for producing a plant having altered lignin composition, comprising:
(a) transforming a plant cell with the construct of any one of claims 2 and 4 to provide a transgenic cell;
(b) expressing said polynucleotide, thereby altering the lignin composition; and
(c) cultivating the transgenic cell under conditions conducive to regeneration and mature plant growth.
13. A method for increasing the amount of an enzyme involved in a lignin biosynthetic pathway in a plant comprising stably incorporating into the genome of the plant the construct of any one of claims 2 and 4 , expressing said polynucleotide, thereby increasing the amount of the encoded enzyme which is involved in a lignin biosynthetic pathway.
14. An isolated polynucleotide that encodes SEQ ID NO:183.
15. An isolated polynucleotide comprising a sequence selected from the group consisting of:
(a) full-length complement of SEQ ID NO: 151;
(b) frill-length reverse complement of SEQ ID NO: 151; and
(c) frill-length reverse sequence of SEQ ID NO: 151.
16. A recombinant genetic construct comprising the polynucleotide of claim 15 .
17. A transgenic cell comprising the recombinant genetic construct of claim 16 .
18. A transgenic plant comprising a transgenic plant cell according to claim 3 , or fruit or seeds or progeny thereof, wherein the fruit, seeds or progeny thereof comprise a recombinant construct comprising SEQ ID NO: 151.