Human coagulation factor VII variants
The present invention relates to novel human coagulation Factor VIIa variants having coagulant activity as well as nucleic acid constructs encoding such variants, vectors and host cells comprising and expressing the nucleic acid, pharmaceutical compositions, uses and methods of treatment.
1. A variant Factor VII polypeptide comprising at least one substitution relative to the sequence of SEQ ID No:1 selected from the group consisting of:
(a) substitution of Val158 with any amino acid other than Ala;
(b) substitution of Glu296 with any amino acid other than Ala and
(c) combinations of any of the foregoing,
wherein the ratio between the activity of the variant and the activity of Factor VII having the sequence of SEQ ID NO:1 is at least about 1.25, when measured in an in vitro hydrolysis assay.
2. A variant as defined in claim 1 comprising two substitutions.
3. A variant as defined in claim 1 further comprising at least one substitution selected from the group consisting of:
(e) substitution of Lys157 with any amino acid other than Ala;
(f) substitution of Lys337 with any amino acid other than Ala;
(g) substitution of Asp334 with any amino acid other than Ala;
(h) substitution of Ser336 with any amino acid other than Ala; and
(i) combinations of any of the foregoing.
4. A variant as defined in claim 3 , wherein Lys157 is replaced with an amino acid selected from the group consisting of Gly, Val, Ser, Thr, Asn, Gln, Asp, and Glu.
5. A variant as defined in claim 3 , wherein Lys337 is replaced with an amino acid selected from the group consisting of Ala, Gly, Val, Ser, Thr, Asn, Gln, Asp, and Glu.
6. A variant as defined in claim 3 , wherein Asp334 is replaced with an amino acid selected from the group consisting of Gly and Glu.
7. A variant as defined in claim 3 , wherein Ser336 is replaced with an amino acid selected from the group consisting of Gly and Glu.
8. A variant as defined in claim 7 , wherein said variant is V158D+E296V+M298Q+K337A-FVII.
9. A variant as defined in claim 1 , wherein Val158 is replaced with an amino acid selected from the group consisting of Ser, Thr, Asn, Gln, Asp, and Glu.
10. A variant as defined in claim 1 , wherein Glu296 is replaced with an amino acid selected from the group consisting of Arg, Lys, and Val.
11. A variant as defined in claim 1 , wherein said substituted amino acid can be encoded by a nucleic acid construct.
12. A variant as defined in claim 1 , wherein said Factor VII polypeptide is human Factor VII.
13. A variant as defined in claim 1 , wherein said Factor VII polypeptide is human Factor VIIa.
14. A variant as defined in claim 1 , further comprising at least one additional substitution of an amino acid corresponding to one of positions 153-406 of SEQ ID NO:1.
15. A variant as defined in claim 1 , further comprising up to 20 additional substitutions of amino acids corresponding to positions 153-406 of SEQ ID NO:1.
16. A variant as defined in claim 1 , further comprising at least one additional subsitution of an amino acid corresponding to one of positions 159-170 of SEQ ID NO:1.
17. A variant as defined in claim 1 , further comprising at least one additional subsitution of an amino acid corresponding to one of positions 290-312 of SEQ ID NO:1.
18. A variant as defined in claim 1 , further comprising at least one additional subsitution of an amino acid corresponding to one of positions 330-339 of SEQ ID NO:1.
19. A variant as defined in claim 1 , wherein the only substitution in said Factor VII polypeptide relative to the sequence of SEQ ID No:1 is selected from the group consisting of:
(a) substitution of Val158 with any different amino acid;
(b) substitution of Glu296 with any different amino acid; and
(c) combinations of any of the foregoing with the proviso that the variant is not V158A-FVII or E296A-FVII.
20. A variant as defined in claim 19 , wherein said variant further comprises substitution of M298 with any other amino acid.
21. A variant as defined in claim 20 , wherein said variant is V158D+E296V+M298Q-FVII.
22. A variant as defined in claim 1 selected from the group consisting of V158T+M298Q-FVII, E296V+M298Q-FVII, V158D+E296V-FVII, V158D+M298Q-FVII, and V158D+M298K-FVII.
23. A variant as defined in claim 1 , wherein said variant is V158D+E296V+M298Q+K337A-FVII.
24. A pharmaceutical composition comprising a variant Factor VII polypeptide as defined in claim 1 and a pharmaceutically acceptable carrier.
25. A method for treatment of bleeding episodes, said method comprising administering to a subject in need of such treatment an effective amount for said treatment of a variant Factor VII polypeptide as defined in claim 1 .
26. A method for enhancing haemostasis, said method comprising administering to a subject in need of such enhancement an effective amount for said enhancement of a variant Factor VII polypeptide as defined in claim 1 .