DNA sequence regulating plant fruit-specific expression
View Patent ↗The present invention has for its object to provide a DNA sequence capable of regulating the expression of a desired gene specifically in the fruit of a plant, a plasmid containing that DNA sequence as well as a plant cell, plant body and microorganism transformed with the plasmid. One aspect of the present invention is constituted by a fruit-specific expression-regulating unit DNA sequence which comprises a region comprising the base sequence shown under SEQ ID NO:1.
1. A fruit-specific expression-regulating unit DNA sequence consisting of the DNA sequence −1181 to −1 of a cucumisin promoter that consists of the base sequence under SEQ ID NO:3 when the 3′ terminus of said base sequence is numbered −1 and the 5′ terminus thereof is numbered −1181.
2. A fruit-specific expression-regulating unit DNA sequence consisting of the DNA sequence −865 to −1 of a cucumisin promoter that consists of the base sequence under SEQ ID NO:3 when the 3′ terminus of said base sequence is numbered −1 and the 5′ terminus thereof is numbered −1181.
3. A fruit-specific expression-regulating unit DNA sequence consisting of the DNA sequence −371 to −1 of a cucumisin promoter that consists of the base sequence under SEQ ID NO:3 when the 3′ terminus of said base sequence is numbered −1 and the 5′ terminus thereof is numbered −1181.
4. A fruit-specific expression-regulating unit DNA sequence consisting of the DNA sequence −310 to −1 of a cucumisin promoter that consists of the base sequence under SEQ ID NO:3 when the 3′ terminus of said base sequence is numbered −1 and the 5′ terminus thereof is numbered −1181.
5. A fruit-specific expression-regulating unit DNA sequence consisting of the DNA sequence −254 to −1 of a cucumisin promoter that consists of the base sequence under SEQ ID NO:3 when the 3′ terminus of said base sequence is numbered −1 and the 5′ terminus thereof is numbered −1181.
6. A fruit-specific expression-regulating unit DNA sequence consisting of the DNA sequence −234 to −1 of a cucumisin promoter that consists of the base sequence under SEQ ID NO:3 when the 3′ terminus of said base sequence is numbered −1 and the 5′ terminus thereof is numbered −1181.
7. A plasmid which comprises a DNA coding for a structural gene product that is expressed in a plant or for a corresponding antisense RNA, a terminator for said DNA, which terminator functions in the plant, and the DNA sequence according to claim 1 for regulating specific expression of said DNA in said plant.
8. A plasmid which comprises a DNA coding for a structural gene product that is expressed in a plant or for a corresponding antisense RNA, a terminator for said DNA, which terminator functions in the plant, and the DNA sequence according to claim 2 for regulating specific expression of said DNA in said plant.
9. A plasmid which comprises a DNA coding for a structural gene product that is expressed in a plant or for a corresponding antisense RNA, a terminator for said DNA, which terminator functions in the plant, and the DNA sequence according to claim 3 for regulating specific expression of said DNA in said plant.
10. A plasmid which comprises a DNA coding for a structural gene product that is expressed in a plant or for a corresponding antisense RNA, a terminator for said DNA, which terminator functions in the plant, and the DNA sequence according to claim 4 for regulating specific expression of said DNA in said plant.
11. A plasmid which comprises a DNA coding for a structural gene product that is expressed in a plant or for a corresponding antisense RNA, a terminator for said DNA, which terminator functions in the plant, and the DNA sequence according to claim 5 for regulating specific expression of said DNA in said plant.
12. A plasmid which comprises a DNA coding for a structural gene product that is expressed in a plant or for a corresponding antisense RNA, a terminator for said DNA, which terminator functions in the plant, and the DNA sequence according to claim 6 for regulating specific expression of said DNA in said plant.
13. A plant cell which is transformed with the plasmid according to claim 7 .
14. A plant cell which is transformed with the plasmid according to claim 8 .
15. A plant cell which is transformed with the plasmid according to claim 9 .
16. A plant cell which is transformed with the plasmid according to claim 10 .
17. A plant cell which is transformed with the plasmid according to claim 11 .
18. A plant cell which is transformed with the plasmid according to claim 12 .
19. A plant body which is transformed with the plasmid according to claim 7 .
20. A plant body which is transformed with the plasmid according to claim 8 .
21. A plant body which is transformed with the plasmid according to claim 9 .
22. A plant body which is transformed with the plasmid according to claim 10 .
23. A plant body which is transformed with the plasmid according to claim 11 .
24. A plant body which is transformed with the plasmid according to claim 12 .
25. A microorganism harboring the plasmid according to claim 7 .
26. A microorganism harboring the plasmid according to claim 8 .
27. A microorganism harboring the plasmid according to claim 9 .
28. A microorganism harboring the plasmid according to claim 10 .
29. A microorganism harboring the plasmid according to claim 11 .
30. A microorganism harboring the plasmid according to claim 12 .