Core-shell metal particles coated with dextran or aminodextran carrying SH groups, and use thereof
View Patent ↗Colloidal metal particles having a coating comprising non cross-linked, SH-activated dextran or aminodextran. Biomolecules may be linked directly or indirectly to the particles, which are useful as detection reagents in immunological assays.
1. A core-shell particle comprising a colloidal metal particle surrounded by a shell wherein the shell comprises non cross-linked dextran or non cross-linked aminodextran wherein said dextran or aminodextran is modified to carry SH groups.
2. The core-shell particle of claim 1 , wherein the metal is a noble metal.
3. The core-shell particle of claim 2 , wherein the noble metal is gold.
4. The core-shell particle of claim 1 , further comprising SH-reactive linker molecules bound to the metal particle via the SH groups.
5. The core-shell particle of claim 4 , wherein the linker molecules are further bound to biomolecules.
6. The core-shell particle of claim 5 , wherein the biomolecules are selected from the group consisting of antibodies, lectin, avidin and streptavidin.
7. The core-shell particle of claim 5 , wherein the biomolecules are antibodies.
8. The core-shell particle of claim 1 , wherein the non cross-linked dextran or non cross-linked aminodextran is further bound to biomolecules.
9. The core-shell particle of claim 1 wherein the shell comprises non cross-linked aminodextran wherein said aminodextran is modified to carry SH groups.
10. The core-shell particle of claim 9 wherein the metal is a noble metal.
11. The core-shell particle of claim 10 wherein the noble metal is gold.
12. The core-shell particle of claim 11 wherein the non cross-linked aminodextran further bound to a biomolecule selected from the group consisting of antibodies, lectin, avidin, and streptavidin.
13. The core-shell particle of claim 10 wherein the aminodextran has an average molecular weight selected from the range of about 30.000 to about 100,000 Da.
14. A method for producing a core-shell particle comprising a colloidal metal particle surrounded by a shell wherein the shell comprises non cross-linked dextran or non cross-linked aminodextran wherein said dextran or aminodextran is modified to carry SH groups, the method comprising:
a) modifying dextran or aminodextran by inserting SH groups, and
b) coating colloidal metal particles with the SH-modified aminodextran from step (a).
15. The method of claim 14 wherein the metal is gold.