Method for producing a microarray
View Patent ↗A microarray obtainable by slicing a block comprising a plurality of linear bodies or through-holes which carry an organism-related substance, in a direction intersecting the longitudinal direction of the linear bodies or through-holes, wherein the linear body and/or block comprise a substance that reduces the self-fluorescence thereof.
1. A method of producing a microarray which comprises:
forming a block which comprises carbon black, fully dispersed throughout the block, that increases a hardness of the block;
after the forming the block, forming a plurality of through-holes in the block via a drill or a laser;
allowing the through-holes to carry a biological substance;
allowing the block to harden; and
slicing the block in a direction intersecting the longitudinal direction of the through-holes, wherein
the biological substance is selected from a group including
(a) a nucleic acid, an amino acid, a saccharide, or a lipid,
(b) a polymeric substance including at least one substance in (a), and
(c) a substance that interacts with a substance in (a) or (b).
2. A method of producing a microarray which comprises:
preparing a block by overlapping perforated boards having a plurality of holes, passing linear bodies carrying a biological substance through each of the plurality of holes in the perforated boards, opening an interval between the perforated boards, and pouring a resin into the interval to immobilize the linear bodies, the resin comprising carbon black fully dispersed throughout the resin, wherein the carbon black increases a hardness of the resin;
allowing the block including the resin to harden; and
slicing the block in a direction intersecting the longitudinal direction of the linear bodies, wherein
the biological substance is selected from a group including
(a) a nucleic acid, an amino acid, a saccharide, or a lipid,
(b) a polymeric substance including at least one substance in (a), and
(c) a substance that interacts with a substance in (a) or (b).
3. A method of producing a microarray which comprises:
preparing a block by overlapping perforated boards having a plurality of holes, passing linear bodies through each of the plurality of holes in the perforated boards, opening an interval between the perforated boards, and pouring a resin into the interval to immobilize the linear bodies, the resin comprising carbon black fully dispersed throughout the resin, wherein the carbon black increases a hardness of the resin;
allowing each of the linear bodies to carry a biological substance,
allowing the block including the resin to harden; and
slicing the block in a direction intersecting the longitudinal direction of the linear bodies, wherein
the biological substance is selected from a group including
(a) a nucleic acid, an amino acid, a saccharide, or a lipid,
(b) a polymeric substance including at least one substance in (a), and
(c) a substance that interacts with a substance in (a) or (b).
4. The method of producing as in claim 2 , wherein the allowing the through-holes to carry the biological substance includes carrying the biological substance via a gel.
5. The method of producing as in claim 2 , further comprising:
soaking a bundle of linear bodies with a biological substance; and
baking the bundle of linear bodies with an ultraviolet light.
6. The method of producing as in claim 3 , wherein
the linear bodies are hollow fibers, and
the allowing each of the linear bodies to carry the biological substance includes introducing the biological substance to a hollow part of the hollow fibers by filling the hollow part with a high molecular polymer including the biological substance.
7. The method of production as in any one of claims 1 - 3 , wherein the carbon black content is 0.5-10% by mass.