Isolated human transporter proteins, nucleic acid molecules, encoding human transporter proteins, and uses thereof
View Patent ↗The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the transporter peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the transporter peptides, and methods of identifying modulators of the transporter peptides.
1. An isolated nucleic acid molecule consisting of a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence that encodes a protein comprising the amino acid sequence of SEQ ID NO:2;
(b) a nucleotide sequence consisting of the nucleic acid sequence of SEQ ID No: 1;
(c) a nucleotide sequence consisting of the nucleic acid sequence of SEQ ID No: 3; and
(d) a nucleotide sequence that is completely complementary to a nucleotide sequence of (a)–(c).
2. A nucleic acid vector comprising a nucleic acid molecule of claim 1 .
3. An isolated host cell comprising the nucleic acid vector of claim 2 .
4. A process for producing a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, comprising culturing the host cell of claim 3 under conditions sufficient for the production of said polypeptide, and recovering the peptide from the host cell culture.
5. An isolated polynucleotide consisting of the nucleotide sequence set forth in SEQ ID NO: 1.
6. An isolated polynucleotide consisting of the nucleotide sequence set forth in SEQ ID NO: 3.
7. A vector according to claim 2 , wherein said vector is selected from the group consisting of a plasmid, virus, and bacteriophage.
8. A vector according to claim 2 , wherein said isolated nucleic acid molecule is inserted into said vector in proper orientation and correct reading frame such that the protein of SEQ ID NO: 2 may be expressed by a cell transformed with said vector.
9. A vector according to claim 8 , wherein said isolated nucleic acid molecule is operatively linked to a promoter sequence.
10. An isolated nucleic acid molecule consisting of a nucleotide sequence that is completely complementary to a nucleotide sequence of
(a) a nucleotide sequence that encodes a protein comprising the amino acid sequence of SEQ ID NO:2;
(b) a nucleotide sequence consisting of the nucleic acid sequence of SEQ ID No: 1; or
(c) a nucleotide sequence consisting of the nucleic acid sequence of SEQ ID No: 3.