IP Library Granted Patent US 7,235,719
Granted Patent B2
US 7,235,719 · App. 10/302,906 · Granted Jun 26, 2007

Cultivated tomato plant having increased brix value and method of producing same

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Quick Facts
Patent No.
US 7,235,719
App. No.
10/302,906
Granted
Jun 26, 2007
Kind
B2
Abstract

A cultivated tomato plant having a genome including an introgression derived from a green-fruited tomato, the green-fruited tomato being characterized by fruits having a brix value above 5 brix units, the introgression including a portion of chromosome 9 of the green-fruited tomato, the portion extending telomerically to a point so as to exclude from the portion an allele of the green-fruited tomato being responsible for an undesired trait selected from the group consisting of higher percent green fruit yield and longer internodes characterizing the green-fruited tomato as is compared to the cultivated tomato plant, the introgression inherently increasing by at least 6% a brix value characterizing fruits of the cultivated tomato plant as is compared to a nearly isogenic tomato plant lacking the introgression.

Claims (27)

1. A cultivated tomato plant of the species Lycopersicon esculentum having a genome comprising an introgression derived from Lycopersicon pennellii, said introgression increasing the Brix value of fruits of said cultivated tomato plant by at least 6% as compared to a plant of the same genotype without the introgression, where said introgression consists essentially of the introgression of tomato plant Lycopersicon pennellii IL 9-2-6.

2. The cultivated tomato plant of claim 1 , wherein fruits of the cultivated tomato plant are characterized by an average fruit mass greater than 30 grams.

3. The cultivated tomato plant of claim 1 , wherein internodes of the cultivated tomato plant are shorter than 15 cm.

4. The cultivated tomato plant of claim 1 , wherein the cultivated tomato is of a determinate or semideterminate tomato line.

5. The cultivated tomato plant of claim 1 , wherein said cultivated tomato is of an indeterminate tomato line.

6. A tomato fruit of the tomato plant of claim 1 .

7. A method of generating a cultivated Lycopersicon esculentum tomato plant having fruits characterized by an increased Brix value, the method comprising the step of introgressing to a genome of said tomato plant an introgression from Lycopersicon pennellii, said introgression increasing the Brix value of fruits of said cultivated tomato plant by at least 6% as compared to a plant of the same genotype without the introgression, where said introgression consists essentially of the introgression of tomato plant Lycopersicon pennellii IL 9-2-6.

8. The method of claim 7 , wherein said fruits of the cultivated tomato plant are characterized by an average fruit mass greater than 30 grams.

9. The method of claim 7 , wherein internodes of the cultivated tomato plant are shorter than 15 cm.

10. The method of claim 7 , wherein the cultivated tomato is of a determinate or semideterminate tomato line.

11. The method of claim 7 , wherein said cultivated tomato is of an indeterminant indeterminate greenhouse tomato line.

12. A tomato fruit derived from the method of claim 7 .

13. A method of generating a tomato plant having fruits characterized by an increased Brix value, the method comprising the steps of:

(a) crossing a first cultivated Lycopersicon esculentum tomato with tomato line Lycopersicon pennellii IL 9-2-6, so as to generate a first hybrid tomato characterized by a fruit Brix value higher than that of said cultivated Lycopersicon esculentum tomato;

(b) crossing said first hybrid tomato or offsprings thereof with a second cultivated Lycopersicon esculentum tomato being characterized by a phenotype different than said first cultivated Lycopersicon esculentum tomato; and

(c) isolating offsprings resulting from step (b) characterized by a phenotype of said second cultivated Lycopersicon esculentum tomato yet with fruits having a higher Brix value than that of fruits of said second cultivated Lycopersicon esculentum tomato.

14. The method of claim 13 , further comprising the step of:

(d) selfing said offsprings resultant from step (c) so as to establish a tomato line characterized by a phenotype of said second cultivated Lycopersicon esculentum tomato yet with fruits having said higher Brix value than that of fruits of said second cultivated Lycopersicon esculentum tomato.

15. A seed of the tomato plant of claim 1 .

16. A method of producing a hybrid, selfed or backcrossed plant, the method comprising the step of crossing the cultivated tomato plant of claim 1 with a cultivated Lycopersicon esculentum tomato plant.

17. A method of producing a tomato seed of the plant of claim 16 , comprising the step of crossing the cultivated tomato plant of claim 1 with a cultivated Lycopersicon esculentum tomato plant and deriving a tomato fruit that has produced said tomato seed.

18. A tomato seed designated L. pennellii IL 9-2-6, a sample of said seed having been deposited under National Collection of Industrial Bacteria number NCIMB 41379.

19. A tomato seed including a genome comprising an introgression derived from Lycopersicon pennellii , said introgression increasing the Brix value of fruits from a tomato plant grown from said tomato seed by at least 6% as compared to a plant of the same genotype without the introgression, where said introgression consists essentially of the introgression of tomato plant Lycopersicon pennellii IL 9-2-6.

20. A tomato plant, or parts thereof produced by growing the seed of claim 19 .

21. Pollen of the plant of claim 1 .

22. A tomato seed produced by growing said tomato plant of claim 20 , wherein the genome of said tomato seed comprises said introgression.

23. A tomato plant, or parts thereof, produced from seed of claim 22 .

Assignments (7)
CHANGE OF NAME Recorded Dec 31, 2012
From: MONSANTO INVEST N.V.
To: MONSANTO INVEST B.V.
Reel/Frame 029547/0285 →
MERGER Recorded Apr 18, 2011
From: DE RUITER SEEDS B.V.; DE RUITER SEEDS R&D B.V.; DE RUITER SEEDS PRODUCTION B.V.; STAGROS AMSTERDAM B.V.
To: MONSANTO INVEST N.V.
Reel/Frame 026140/0451 →
MERGER Recorded Mar 22, 2011
From: DE RUITER SEEDS B.V.; DE RUITER SEEDS R&D B.V.; DE RUITER SEEDS PRODUCTION B.V.; STAGROS AMSTERDAM B.V.
To: MONSANTO INVEST B.V.
Reel/Frame 025994/0132 →
SECURITY AGREEMENT Recorded Nov 6, 2008
From: DE RUITER SEEDS R&D B.V.
To: ABN AMRO BANK N.V.
Reel/Frame 021794/0001 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 14, 2007
From: DE RUITER SEEDS B.V.
To: DE RUITER SEEDS R&D B.V.
Reel/Frame 018888/0047 →
MERGER Recorded Feb 14, 2007
From: DE RUITER SEEDS C.V., A LIMITED PARTNERSHIP
To: DE RUITER SEEDS B.V.
Reel/Frame 018888/0015 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 25, 2002
From: ZAMIR, DANI; PLEBAN, TZILI; FRIDMAN, EYAL
To: DE RUITER SEEDS C.V. LP
Reel/Frame 013545/0138 →