Nucleic acids encoding TRAIN-R: a cysteine rich member of the TNF-receptor family
View Patent ↗The present invention relates to novel receptors in the TNF family. A novel receptor has been identified, referred to herein as TRAIN. The invention relates to DNA sequences encoding TRAIN receptors. The invention also contemplates recombinant DNAs comprising a sequence encoding TRAIN receptors or fragments thereof, as well as hosts with stably integrated TRAIN-R sequences introduced into their genome, or possessing episomal elements.
1. An isolated nucleic acid encoding a TRAIN receptor polypeptide, wherein said nucleic acid comprises the nucleotide sequence of SEQ ID NO: 7.
2. An isolated nucleic acid encoding a TRAIN receptor polypeptide, wherein said polypeptide comprises an amino acid sequence selected from the group consisting of:
(a) SEQ ID NO: 3;
(b) SEQ ID NO: 9; and
(c) amino acid residues 26 to 417 of SEQ ID NO:3;
(d) amino acid residues 1 to 173 of SEQ ID NO:3;
(e) amino acid residues 26 to 173 of SEQ ID NO:3;
(f) amino acid residues 1 to 190 of SEQ ID NO:3;
(g) amino acid residues 26 to 190 of SEQ ID NO:3;
(h) amino acid residues 174 to 190 of SEQ ID NO:3;
(i) amino acid residues 174 to 417 of SEQ ID NO:3; and
(j) amino acid residues 191 to 417 of SEQ ID NO:3.
3. A vector comprising the isolated nucleic acid of claim 1 , wherein said nucleic acid is operatively linked to an expression control sequence capable of expressing said isolated nucleic acid in a cell.
4. An isolated cell transformed with the vector of claim 3 , wherein said cell expresses said TRAIN receptor polypeptide.
5. A vector comprising the isolated nucleic acid of claim 2 , wherein said nucleic acid is operatively linked to an expression control sequence capable of expressing said isolated nucleic acid in a cell.
6. An isolated cell transformed with the vector of claim 5 , wherein said cell expresses said TRAIN receptor polypeptide.
7. A method of producing a TRAIN receptor polypeptide, comprising the steps of:
(a) constructing a vector comprising an isolated nucleic acid having the nucleic acid sequence of SEQ ID NO:7 operatively linked to an expression control sequence;
(b) transforming a host cell with said vector, thereby creating a transformed cell; and
(c) growing said transformed cell in cell medium, whereby said TRAIN receptor polypeptide is expressed.
8. The method according to claim 7 , further comprising the step of isolating said TRAIN receptor polypeptide from said cell medium.
9. The method according to claim 7 , wherein said host cell is selected from the group consisting of yeast, insect, bacterial and mammalian cells.
10. The method according to claim 7 , wherein said vector comprises a fragment of said isolated DNA encoding a polypeptide having an amino acid sequence selected from the group consisting of:
(a) SEQ ID NO: 3;
(b) SEQ ID NO: 9; and
(c) amino acid residues 26 to 417 of SEQ ID NO:3;
(d) amino acid residues 1 to 173 of SEQ ID NO:3;
(e) amino acid residues 26 to 173 of SEQ ID NO:3;
(f) amino acid residues 1 to 190 of SEQ ID NO:3;
(g) amino acid residues 26 to 190 of SEQ ID NO:3;
(h) amino acid residues 174 to 190 of SEQ ID NO:3;
(i) amino acid residues 174 to 417 of SEQ ID NO:3; and
(j) amino acid residues 191 to 417 of SEQ ID NO:3.
11. The method according to claim 10 , further comprising the step of isolating said TRAIN receptor polypeptide from said cell medium.
12. The method according to claim 10 , wherein said host cell is selected from the group consisting of yeast, insect, bacterial and mammalian cells.