HoxD3 and HoxA3 compositions and methods for improved wound healing
View Patent ↗The present invention provides methods and compositions useful in localized transfer of genetic material or proteins. Moreover, the present invention provides methods and compositions for improving and/or controlling wound healing by applying a wound care device comprising HoxD3 and/or HoxA3. In addition, the present invention provides methods and compositions for improved wound healing in subjects having impaired healing capabilities, such as diabetic subjects.
1. A method comprising:
a) providing;
i) a subject with a diabetic skin wound, and
ii) a composition comprising a gene delivery vehicle and an expression vector comprising HoxA3 nucleic acid encoding a HoxA3 protein, wherein said HoxA3 protein is the protein set forth in SEQ ID NO:15 or a biologically active variant thereof that differs by less than 1%; and
b) applying said composition to said wound under conditions suitable for transfecting at least one cell of said wound with said expression vector.
2. The method of claim 1 , wherein said HoxA3 protein is a human HoxA3 protein set forth in SEQ ID NO:15.
3. The method of claim 1 , wherein said applying is under conditions such that wound healing is accelerated.
4. The method of claim 1 , wherein said applying is under conditions such that wound closure is accelerated.
5. The method of claim 1 , wherein said applying is under conditions such that angiogenesis in said wound is enhanced.
6. The method of claim 1 , wherein said applying is under conditions such that type I collagen expression in said wound is enhanced.
7. The method of claim 1 , wherein said composition further comprises a cellulosic material.
8. The method of claim 1 , wherein said composition is located in a wound care device.
9. The method of claim 1 , wherein said skin wound has a reduced level of expression of HoxD3.
10. The method of claim 1 , wherein said wound is an ulcer.
11. The method of claim 1 , wherein said expression vector further comprises HoxD3 nucleic acid encoding a HoxD3 protein, wherein said HoxD3 protein comprises a protein set forth in SEQ ID NO:2 or a biologically active variant thereof that differs by less than 1%.
12. The method of claim 1 , wherein said HoxA3 nucleic acid comprises a nucleic acid set forth in SEQ ID NO:14.
13. The method of claim 11 , wherein said HoxD3 protein is a human HoxD3 protein set forth in SEQ ID NO:2.
14. The method of claim 11 , wherein said HoxD3 nucleic acid comprises a nucleic acid set forth in SEQ ID NO:1.
15. The method of claim 1 , wherein said gene delivery vehicle is selected from the group consisting of gel matrices, liposomes, virosomes, cationic lipids, polylysine, adenoviral vectors, retroviral vectors and gold particles.
16. A method comprising:
a) providing;
i) a subject with a diabetic skin wound, and
ii) a composition comprising a cellulosic material and an expression vector comprising HoxA3 nucleic acid encoding the HoxA3 protein set forth in SEQ ID NO:15; and
b) applying said composition to said wound under conditions suitable for transfecting at least one cell of said wound with said expression vector.
17. The method of claim 16 , wherein said cellulosic material comprises methyl cellulose pellets comprising said expression vector.
18. The method of claim 17 , wherein said HoxA3 nucleic acid comprises a nucleic acid set forth in SEQ ID NO:14.