Compositions and methods of selective nucleic acid isolation
View Patent ↗The invention relates to methods for isolating and/or identifying nucleic acids. The invention also provides kits for isolating and/or identifying nucleic acids.
1. A method of isolating DNA from a biological sample comprising:
selectively binding DNA to a solid phase by contacting the biological sample with the solid phase under conditions which selectively bind DNA, wherein the DNA selectively bound to the solid phase is genomic DNA, and wherein the conditions which selectively bind DNA comprise using a binding buffer comprising an alkaline pH above 8.0;
separating the solid phase with the bound DNA from an unbound portion of the biological sample;
and isolating the DNA from the solid phase.
2. The method of claim 1 , wherein the isolating the DNA from the solid phase comprises eluting the DNA.
3. The method of claim 1 , wherein the solid phase is a siliceous material.
4. The method of claim 3 , wherein the siliceous material is selected from at least one of silica, silica dioxide, diatomaceous earth, glass, Celite, and silica gel.
5. The method of claim 4 , wherein the solid phase is in a form selected from at least one of a particle, a bead, a membrane, a frit, a surface within a chamber, and a side of a container.
6. The method of claim 1 , wherein the binding buffer further comprises a large anion, wherein the large anion is at least as large as a bromide ion.
7. The method of claim 6 , wherein the large anion is selected from at least one of picrate, tannate, tungstate, molybdate, perchiorate, and sulfosalicylate.
8. The method of claim 6 , wherein the large anion is selected from at least one of trichloroacetate, tribromoacetate, thiocyanate, and nitrate.
9. The method of claim 6 , wherein the large anion is selected from at least one of iodide and bromide.
10. The method of claim 1 , wherein the alkaline pH is equal to, or above 9.0.
11. The method of claim 1 , wherein the alkaline pH is equal to, or above 10.0.
12. A method of identifying DNA in a biological sample comprising:
selectively binding DNA to a solid phase by contacting the biological sample with the solid phase under conditions which selectively bind DNA, wherein the DNA selectively bound to the solid phase is capable of being subsequently isolated from the solid phase, wherein the DNA selectively bound to the solid phase is genomic DNA, and wherein the conditions which selectively bind DNA comprise using a binding buffer comprising an alkaline pH above 8.0;
separating the solid phase with the bound DNA from an unbound portion of the biological sample; and
identifying the DNA bound to the solid phase.
13. The method of claim 12 , wherein the identifying the DNA bound to the solid phase comprises amplifying the DNA bound to the solid phase.