Plant regulatory sequences for selective control of gene expression
View Patent ↗The present invention relates to nucleic acid sequences for regulating gene expression in plants. In particular, the invention relates to 5′ regulatory sequences which are useful for regulating expression of heterologous DNAs in plants and methods for identifying multiple 5′ regulatory sequences which confer a particular expression profile when operably linked to DNA sequences. The invention also relates to expression vectors containing the 5′ regulatory sequences and to transgenic plants containing the expression vectors.
1. An isolated DNA molecule comprising SEQ ID NO:51.
2. A recombinant DNA construct comprising a promoter polynucleotide comprising SEQ ID NO:51, wherein said polynucleotide is operably linked to a second polynucleotide encoding a protein of interest and a 3′ non-translated region.
3. A transgenic plant or plant cell comprising the DNA construct of claim 2 .
4. A transgenic plant of claim 3 , wherein said plant is a monocot plant.
5. A method of making a transgenic plant comprising:
(i) introducing into a plant cell a DNA construct comprising
(a) a promoter comprising the DNA molecule of claim 1 , wherein the promoter is operably linked to
(b) a second polynucleotide encoding a protein of interest and
(c) a 3′ non-translated region;
(ii) selecting said plant cell; and
(iii) regenerating said plant cell into a plant.