Chandra: a Th1-specific protein
View Patent ↗The present invention provides nucleic acid and protein sequences for a novel Th-1 cell-specific protein, Chandra. The herein-disclosed sequences can be used for any of a number of purposes, including for the specific detection of Th1 lymphocytes, for the identification of molecules that associate with and/or modulate the activity of Chandra, to diagnose any of a number of conditions associated with Th1 or Th2 cell activity, or to modulate the number and/or activity of Th1 or Th2 lymphocytes in a mammal.
1. An isolated polypeptide having at least about 95% amino acid sequence identity to SEQ ID NO:1 over the full-length of SEQ ID NO:1, and said polypeptide possessing the ability to inhibit IL-4 dependent signalling.
2. The polypeptide of claim 1 , wherein said polypeptide specifically binds to polyclonal antibodies generated against a polypeptide comprising an amino acid sequence of SEQ ID NO:1.
3. The polypeptide of claim 1 , wherein said polypeptide comprises an amino acid sequence of SEQ ID NO:1.
4. The polypeptide of claim 1 , wherein said polypeptide is expressed in Th1 lymphocytes.
5. The polypeptide of claim 1 , wherein said polypeptide inhibits IL-4 activity in Th2 cells.
6. The polypeptide of claim 1 , wherein said polypeptide inhibits Th2 cell differentiation and production.
7. The polypeptide of claim 1 , wherein said polypeptide is a polymorphic variant of SEQ ID NO:1, wherein an isoleucine residue is substituted for a valine residue at amino acid position 11.
8. The polypeptide of claim 1 , wherein said polypeptide is a polymorphic variant of SEQ ID NO:1, wherein an alanine residue is substituted for a glycine residue at amino acid position 3.
9. An isolated polypeptide having the amino acid sequence of SEQ ID NO:1.
10. The polypeptide of claim 1 , wherein said polypeptide promotes Th1 cell differentiation and production.