Treating anemia in subjects by administration of plasmids encoding growth hormone releasing hormone
View Patent ↗The present invention pertains to compositions and methods for plasmid-mediated supplementation. The compositions and method are useful for retarding the growth of the tumor, and retarding cachexia, wasting, anemia and other effects that are commonly associated in cancer bearing animals. Overall, the embodiments of the invention can be accomplished by delivering an effective amount of a nucleic acid expression construct that encodes a GHRH or functional biological equivalent thereof into a tissue of an animal and allowing expression of the encoded. gene in the animal. For example, when such a nucleic acid sequence is delivered into the specific cells of the tissue specific constitutive expression is achieved.
1. A method of treating anemia in a patient, the anemia being caused by a decrease in red blood cell production, comprising:
(a) administering into the muscle of the subject an effective amount of a plasmid expression construct encoding HV-GHRH (SEQ ID NO: 1), wherein the encoded HV-GHRH is operably linked to a SPc5-12 promoter of the plasmid expression construct, and wherein the plasmid expression construct is mixed with a transfection-facilitating polypeptide;
(b) electroporating the muscle having the effective amount of plasmid expression construct encoding the HV-GHRH (SEQ ID NO: 1),
wherein the subject is a domesticated animal or human and wherein the HV-GHRH is biologically active and expressed in the muscle cells and released by the muscle cells causing a facilitation of growth hormone secretion and thereby causing increased red blood cell production, thereby treating the anemia.