Apparatus and method for synthesizing combinatorial libraries
View Patent ↗An apparatus and method for synthesizing a combinatorial library comprising a plurality of chemical compounds such that the chemical composition of each compound is easily tracked. The library compounds are synthesized on solid-phase supports, which are spatially arranged in frames during synthesis according to a predetermined protocol, such that each solid-phase support passes through a series of unique spatial 2D or 3D addresses by which the chemical composition of each compound may be determined at any point during synthesis. Solid-phase supports include hollow tubular-shaped lanterns and gears.
1. An apparatus for synthesizing a combinatorial library comprising:
a 3-D array of solid-phase supports comprised of a plurality of 2-D frames with means to permit stacking into the 3-D array of solid-phase supports;
wherein the 2-D frames have a plurality of intersecting double-drilled holes formed from first and second adjacent holes, said first hole receiving said solid-phase support in a friction fit attachment and said second hole providing a channel means for delivering reagents to a subset of the 3-D array of solid-phase supports;
and means for removing said solid-phase supports, without disassembling the 3D array.
2. The apparatus of claim 1 further comprising a channel created through solid-phase supports, which are aligned when 2-D frames are stacked.
3. The apparatus of claim 2 wherein the channel means is provided by aligned second holes provided in the stacked 2-D frames.
4. The apparatus of claim 1 wherein, said double-drilled hole aligned with other double-drilled holes of stacked frames to define a channel.
5. The apparatus of claim 4 wherein the means for removing the solid-phase supports without disassembling the 2-D frames is a device to pop out a column of friction fitted solid supports through the channel defined by the alignment of holes in the 2-D frames in which holes the solid-phase supports are held.
6. The apparatus of claim 1 wherein the solid-phase supports are attached to the frame with cuttable thin connectors.
7. The apparatus of claim 1 for synthesizing a combinatorial library further comprising:
a plurality of reactors for holding reagents, said reactors sized to allow said 3-D array of solid-phase support to be completely immersed in a reagent.
8. The apparatus of claim 7 wherein the solid-phase support are lanterns, having holes in the middle.
9. The apparatus of claim 8 wherein the lanterns are made of polypropylene with a surface of polystyrene and having a tubular structure with an outer diameter of from about 4.0 mm to about 6.0 mm and an inner bore diameter of from about 2.0 mm to about 3.0 mm.
10. The apparatus of claim 9 wherein the outer diameter of each lantern is about 5.0 mm, and the inner bore diameter is about 2.5 mm.