Open-field serial to parallel converter
View Patent ↗Microfluidic devices and systems for affecting the serial to parallel conversion of materials introduced into the device or system. Material or materials to be converted from a serial orientation, e.g., a single channel, into a parallel orientation, e.g., multiple channels, are introduced into an open chamber or field in which containing flows of materials maintain the cohesiveness of the sample material plugs serially introduced into the open chamber. The sample material or materials are then redirected in the chamber toward and into a plurality of parallel channels that also communicate with the chamber.
1. A method of converting a serial flow of materials into a parallel flow of materials, the method comprising:
providing a body having disposed therein an open field chamber having at least first and second sides opposite one another and a third side not opposite either the first or second side, wherein a first plurality of channels is fluidly connected to the first side of the chamber at periodic intervals, wherein a second plurality of channels is fluidly connected to the second side of the chamber at periodic intervals, and wherein a sample introduction channel and a plurality of containing flow channels are fluidly connected to the third side of the chamber;
serially introducing a plurality of discrete quantities of one or more sample materials into the chamber from the sample introduction channel; and
directing flow of the discrete quantities of sample material from the chamber into the second plurality of channels, at least a subset of the second plurality of channels being parallel.
2. The method of claim 1 , wherein the step of directing flow of the discrete quantities of one or more sample materials into the second plurality of channels comprises directing flow of a carrier material from each of the first plurality of channels into the chamber.
3. The method of claim 1 , wherein the step of directing flow of the discrete quantities of one or more sample materials into the second plurality of channels comprises applying a potential difference between the first plurality of channels and the second plurality of channels through the chamber.
4. The method of claim 3 , wherein at least the subset of the second plurality of channels has disposed therein a separation medium, and further comprising separating the sample material in the subset of the second plurality of channels into one or more constituent elements.
5. The method of claim 1 , wherein the step of directing flow of the discrete quantities of one or more sample materials comprises applying a pressure difference between the first plurality of channels and the second plurality of channels through the chamber.
6. The method of claim 1 , wherein the introducing step comprises introducing a plurality of discrete quantities of the same sample material into the chamber.
7. The method of claim 1 , wherein the introducing step comprises introducing a plurality of discrete quantities of different sample materials into the chamber.