Haptens, immunogens and antibodies to oxycodone and its metabolites
View Patent ↗Novel haptens, which can be conjugated to form immunogens, are of formula I, II or III wherein R is a divalent alkyl, cycloalkyl or aryl group having 1 to 10 carbon atoms, and X is a functional group.
1. A compound of formula
wherein R is a divalent alkyl, cycloalkyl or aryl group having 1 to 10 carbon atoms, and X is a functional group.
2. A compound according to claim 1 , wherein R is C 1-3 alkylene.
3. A compound according to claim 1 , wherein X is COOH.
4. A compound according to claim 1 , which is N-[S-(carboxyethyl)thiopropyl]noroxycodone.
5. An immunogen comprising a compound according to claim 1 , coupled to an antigenic carrier.
6. An antibody raised against an immunogen according to claim 5 , and capable of binding with at least one epitope of oxycodone and its metabolites.
7. A conjugate comprising a compound according to claim 1 covalently bound to a detectable label.
8. A method for detecting or determining oxycodone and its metabolites in a sample, which comprises contacting the sample with a conjugate comprising a compound according to claim 1 covalently bound to a detectable label and an antibody raised against an immunogen comprising the compound of claim 1 coupled to an antigenic carrier, and being capable of binding with at least one epitope of oxycodone and its metabolites, and detecting bound conjugate.
9. A kit for determining the presence or amount of oxycodone and its metabolites in a sample, which includes a conjugate comprising a compound according to claim 1 covalently bound to a detectable label and an antibody raised against an immunogen comprising the compound of claim 1 coupled to an antigenic carrier, and being capable of binding with at least one epitope of oxycodone and its metabolites.
10. A compound according to claim 2 , wherein X is COOH.
11. An immunogen comprising a compound according to claim 2 , coupled to an antigenic carrier.
12. An immunogen comprising a compound according to claim 3 , coupled to an antigenic carrier.
13. An immunogen comprising a compound according to claim 4 , coupled to an antigenic carrier.
14. A conjugate comprising a compound according to claim 2 covalently bound to a detectable label.
15. A conjugate comprising a compound according to claim 3 covalently bound to a detectable label.
16. A conjugate comprising a compound according to claim 4 covalently bound to a detectable label.