Methods and compositions for diagnosing and monitoring transplant rejection
View Patent ↗Methods of diagnosing or monitoring transplant rejection, particularly cardiac transplant rejection, in a patient by detecting the expression level of one or more genes in a patient, are described. Diagnostic oligonucleotides for diagnosing or monitoring transplant rejection, particularly cardiac transplant rejection and kits or systems containing the same are also described.
1. A method of diagnosing or monitoring transplant rejection in a patient, comprising detecting the expression level of a nucleic acid in said patient to diagnose or monitor transplant rejection in said patient wherein said nucleic acid comprises the nucleotide sequence SEQ ID NO: 2709.
2. The method of claim 1 wherein said transplant rejection is cardiac transplant rejection.
3. The method of claim 1 wherein said expression level is detected by measuring the RNA level expressed by said nucleic acid.
4. The method of claim 3 , further including isolating RNA from said patient prior to detecting said RNA level expressed by said nucleic acid.
5. The method of claim 3 wherein said RNA level is detected by PCR.
6. The method of claim 3 wherein said RNA level is detected by hybridization.
7. The method of claim 3 wherein said RNA level is detected by hybridization to an oligonucleotide.
8. The method of claim 7 wherein said oligonucleotide comprises DNA, RNA, cDNA, PNA, genomic DNA, or synthetic oligonucleotides.
9. A method of diagnosing or monitoring transplant rejection in a patient, comprising detecting the expression level of a nucleic acid in said patient to diagnose or monitor transplant rejection in said patient wherein said nucleic acid comprises the nucleotide sequence SEQ ID NO: 4647.
10. The method of claim 9 wherein said transplant resection is cardiac transplant rejection.
11. The method of claim 9 wherein said expression level is detected by measuring the RNA level expressed by said nucleic acid.
12. The method of claim 11 , further including isolating RNA from said patient prior to detecting said RNA level expressed by said nucleic acid.
13. The method of claim 11 wherein said RNA level is detected by PCR.
14. The method of claim 11 wherein said RNA level is detected by hybridization.
15. The method of claim 11 wherein said RNA level is detected by hybridization to an oligonucleotide.
16. The method of claim 15 wherein said oligonucleotide comprises DNA, RNA, cDNA, PNA, genomic DNA, or synthetic oligonucleotides.
17. A method of diagnosis or monitoring transplant rejection in a patient, comprising detecting the expression level of a nucleic acid in said patient to diagnose or monitor transplant rejection in said patient wherein said nucleic acid comprises the nucleotide sequence SEQ ID NO: 4685.
18. The method of claim 17 wherein said transplant rejection is cardiac transplant rejection.
19. The method of claim 17 wherein said expression level is detected by measuring the RNA level expressed by said nucleic acid.
20. The method of claim 19 , further including isolating RNA from said patient prior to detecting said RNA level expressed by said nucleic acid.
21. The method of claim 19 wherein said RNA level is detected by PCR.
22. The method of claim 19 wherein said RNA level is detected by hybridization.
23. The method of claim 19 wherein said RNA level is detected by hybridization to an oligonucleotide.
24. The method of claim 23 herein said oligonucleotide comprises DNA, RNA, cDNA, PNA, genomic DNA, or synthetic oligonucleotides.
25. A method of diagnosing or monitoring transplant rejection in a patient, comprising detecting the expression level of a nucleic acid in said patient to diagnose or monitor transplant rejection in said patient wherein said nucleic acid comprises the nucleotide sequence SEQ ID NO: 6489.
26. The method of claim 25 wherein said transplant rejection is cardiac transplant rejection.
27. The method of claim 25 wherein said expression level is detected by measuring the RNA level expressed by said nucleic acid.
28. The method of claim 27 , further including isolating RNA from said patient prior to detecting said RNA level expressed by said nucleic acid.
29. The method of claim 27 wherein said RNA level is detected by PCR.
30. The method of claim 27 wherein said RNA level is detected by hybridization.
31. The method of claim 27 wherein said RNA level is detected by hybridization to an oligonucleotide.
32. The method of claim 31 wherein said oligonucleotide comprises DNA, RNA, cDNA, PNA, genomic DNA, or synthetic oligonucleotides.
33. A method of diagnosing or monitoring transplant rejection in a patient, comprising detecting the expression level of a nucleic acid in said patient to diagnose or monitor transplant rejection in said patient wherein said nucleic acid comprises the nucleotide sequence SEQ ID NO: 4866.
34. The method of claim 33 wherein said transplant rejection is cardiac transplant rejection.
35. The method of claim 33 wherein said expression level is detected by measuring the RNA level expressed by said nucleic acid.
36. The method of claim 35 , further including isolating RNA from said patient prior to detecting said RNA level expressed by said nucleic acid.
37. The method of claim 35 wherein said RNA level is detected by PCR.
38. The method of claim 35 wherein said RNA level is detected by hybridization.
39. The method of claim 35 wherein said RNA level is detected by hybridization to an oligonucleotide.
40. The method of claim 39 wherein said oligonucleotide comprises DNA, RNA, cDNA, PNA, genomic DNA, or synthetic oligonucleotides.
41. A method of diagnosing or monitoring transplant rejection in a patient, comprising detecting the expression level of a nucleic acid in said patient to diagnose or monitor transplant rejection in said patient wherein said nucleic acid comprises the nucleotide sequence SEQ ID NO: 2009.
42. The method of claim 41 wherein said transplant rejection is cardiac transplant rejection.
43. The method of claim 41 wherein said expression level is detected by measuring the RNA level expressed by said nucleic acid.
44. The method of claim 43 further including isolating RNA from said patient prior to detecting said RNA level expressed by said nucleic acid.
45. The method of claim 43 wherein said RNA level is detected by PCR.
46. The method of claim 43 wherein said RNA level is detected by hybridization.
47. The method of claim 43 wherein said RNA level is detected by hybridization to an oligonucleotide.
48. The method of claim 47 wherein said oligonucleotide comprises DNA, RNA, cDNA, PNA, genomic DNA, synthetic oligonucleotides.
49. A method of diagnosing or monitoring transplant rejection in a patient, comprising detecting the expression level of a nucleic acid in said patient to diagnose or monitor transplant reaction in said patient wherein said nucleic acid comprises the nucleotide sequence SEQ ID NO: 1981.
50. The method of claim 49 wherein said transplant rejection is cardiac transplant rejection.
51. The method of claim 49 wherein said expression level is detected by measuring the RNA level expressed by said nucleic acid.
52. The method of claim 51 , further including isolating RNA from said patient prior to detecting said RNA level expressed by said nucleic acid.
53. The method of claim 51 wherein said RNA level is detected by PCR.
54. The method of claim 51 wherein said RNA level is detected by hybridization.
55. The method of claim 51 wherein said RNA level is detected by hybridization to an oligonucleotide.
56. The method of claim 55 wherein said oligonucleotide comprises DNA, RNA, cDNA, PNA, genomic DNA, or synthetic oligonucleotides.
57. A method of diagnosing or monitoring transplant rejection in a patient, comprising detecting the expression level of a nucleic acid in said patient to diagnose or monitor transplant rejection in said patient wherein said nucleic acid comprises the nucleotide sequence SEQ ID NO: 2336.
58. The method of claim 57 wherein said transplant rejection is cardiac transplant rejection.
59. The method of claim 57 wherein said expression level is detected by measuring the RNA level expressed by said nucleic acid.
60. The method of claim 59 , further including isolating RNA from said patient prior to detecting said RNA level expressed by said nucleic acid.
61. The method of claim 59 wherein said RNA level is detected by PCR.
62. The method of claim 59 wherein said RNA level is detected by hybridization.
63. The method of claim 59 wherein said RNA level is detected by hybridization to an oligonucleotide.
64. The method of claim 63 wherein said oligonucleotide comprises DNA, RNA, cDNA, PNA, genomic DNA, or synthetic oligonucleotides.
65. A method of diagnosing or monitoring transplant rejection in a patient, comprising detecting the expression level of a nucleic acid in said patient to diagnose or monitor transplant rejection in said patient wherein said nucleic acid comprises the nucleotide sequence SEQ ID NO: 4946.
66. The method of claim 65 wherein said transplant rejection is cardiac transplant rejection.
67. The method of claim 65 wherein said expression level is detected by measuring the RNA level expressed by said nucleic acid.
68. The method of claim 67 , further including isolating RNA from said patient prior to detecting said RNA level expressed by said nucleic acid.
69. The method of claim 67 wherein said RNA level is detected by PCR.
70. The method of claim 67 wherein said RNA level is detected by hybridization.
71. The method of claim 67 wherein said RNA level is detected by hybridization to an oligonucleotide.
72. The method of claim 71 wherein said oligonucleotide comprises DNA, RNA, cDNA, PNA, genomic DNA, or synthetic oligonucleotides.
73. A method of diagnosing or monitoring transplant rejection in a patient, comprising detecting the expression level of a nucleic acid in said patient to diagnose or monitor transplant rejection in said patient wherein said nucleic acid comprises the nucleotide sequence SEQ ID NO: 2863.
74. The method of claim 73 wherein said transplant rejection is cardiac transplant rejection.
75. The method of claim 73 wherein said expression level is detected by measuring the RNA level expressed by said nucleic acid.
76. The method of claim 75 , further including isolating RNA from said patient prior to detecting said RNA level expressed by said nucleic acid.
77. The method of claim 75 wherein said RNA level is detected by PCR.
78. The method of claim 75 wherein said RNA level is detected by hybridization.
79. The method of claim 75 wherein said RNA level is detected by hybridization to an oligonucleotide.
80. The method of claim 79 wherein said oligonucleotide comprises DNA, RNA, cDNA, PNA, genomic DNA, or synthetic oligonucleotides.