IP Library › Granted Patent US 6,919,194
Granted Patent B2
US 6,919,194 · App. 10/338,731 · Granted Jul 19, 2005

Method for cloning and expression of Tth111II restriction endonuclease-methylase in E. coli

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Quick Facts
Patent No.
US 6,919,194
App. No.
10/338,731
Granted
Jul 19, 2005
Kind
B2
Abstract

The present invention relates to recombinant DNA which encodes the Tth111II restriction endonuclease-methylase fusion protein (Tth111IIRM), expression of Tth111II restriction endonuclease-methylase fusion protein in E. coli cells containing the recombinant DNA, and purification of Tth111II endonuclease-methylase fusion protein to near homogeneity.

Claims (5)

1. Isolated DNA coding for the Tth111II restriction endonuclease-methylase, wherein the isolated DNA is obtainable from Thermus thermophilus 111.

2. A recombinant DNA vector comprising a vector into which a DNA segment encoding the Tth111II restriction endonuclease-methylase gene has been inserted.

3. Isolated DNA encoding the Tth111II restriction endonuclease-methylase, wherein the isolated DNA is obtainable from ATCC No. PTA 4891.

4. A host cell transformed by the vector of claim 2 .

5. A method of producing recombinant Tth111II restriction endonuclease-methylase fusion protein comprising culturing a host cell transformed with the vector of claim 2 under conditions suitable for expression of said endonuclease-methylase.

Assignments (4)
TERMINATION AND RELEASE OF SECURITY INTEREST IN PATENTS Recorded Sep 20, 2023
From: BANK OF AMERICA, N.A. (AS SUCCESSOR-BY-MERGER WITH FLEET NATIONAL BANK)
To: NEW ENGLAND BIOLABS, INC.
Reel/Frame 064962/0066 →
RELEASE PREVIOUSLY RECORDED AT REEL/FRAME 014220/0763 Recorded Oct 22, 2004
From: FLEET NATIONAL BANK
To: NEW ENGLAND BIOLABS, INC.
Reel/Frame 015286/0792 →
SECURITY AGREEMENT Recorded Dec 30, 2003
From: NEW ENGLAND BIOLABS, INC.
To: FLEET NATIONAL BANK
Reel/Frame 014220/0763 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 8, 2003
From: ZHU, ZHENYU; ROBINSON, DEREK; BENNER, JACK; XU, SHUANG-YONG
To: NEW ENGLAND BIOLABS, INC.
Reel/Frame 013650/0442 →