Cell surface molecule-induced macrophage activation
View Patent ↗This invention provides compositions, such as capsules, comprising transformed cells that express immunostimulatory cell surface polypeptides which are expressed on the cell surface and are capable of stimulating an immune response against the cell in a host.
1. A composition comprising:
(a) a core comprising a transformed cell containing a recombinant polynucleotide comprising a promoter operably linked with a polynucleotide sequence encoding a fusion protein comprising the Fc portion of an IgG molecule linked to a cell surface protein, selected from the group consisting of transferrin, CD10, CD13, CD23, CD26, CD38, CD71, CD72, CD74, lymphocyte activation antigen 4F2, B-lymphocyte differentiation antigen BP-1, endoglin cell surface antigen, natural killer cell lectin-like Ly-49, macrophage asialoglycoprotein binding protein, natural killer cell lectin-like receptor NKG2A, natural killer cell lectin-like receptor NKR-PI, and plasma cell membrane antigen PC-1, which anchors the fusion protein to the cell surface, such that, upon expression of the fusion protein on the celIsurface, the Fc portion of the fusion protein is capable of stimulating an immune response against the cell in a host wherein said stimulation occurs without complement fixation; and
(b) a jacket surrounding the core, the jacket comprising a permselective membrane.
2. The composition of claim 1 , wherein said immune response is the activation of phagocytes.
3. A composition comprising:
(a) a core comprising a transformed cell containing a recombinant polynucleotide comprising a promoter operably linked with a polynucleotide sequence encoding a fusion protein comprising the Fc portion of an IgG molecule linked at the amino terminus to the transferrin receptor hinge region wherein upon expression of the fusion protein on the cell surface, the Fc portion of the fusion protein is capable of stimulating an immune response against the cell in a host wherein said stimulation occurs without complement fixation; and
(b) a jacket surrounding the core, the jacket comprising a permselective membrane.
4. The composition of claim 3 , wherein said immune response is the activation of phagocytes.
5. A composition comprising:
(a) a core comprising a transformed cell containing a recombinant polynucleotide comprising a promoter operably linked with a polynucleotide sequence encoding a fusion protein comprising the Fc portion of an IgG molecule linked at the amino terminus to the transferrin receptor hinge region, wherein upon expression, the fusion protein is expressed on the cell surface; and
(b) a jacket surrounding the core, the jacket comprising a permselective membrane.