Hydrolytically degradable carbamate derivatives of poly(ethylene glycol)
View Patent ↗Poly(ethylene glycol) carbamate derivatives useful as water-soluble prodrugs are disclosed. These degradable poly(ethylene glycol) carbamate derivatives also have potential applications in controlled hydrolytic degradation of hydrogels. In such degradable hydrogels, drugs may be trapped in the gel and released by diffusion as the gel degrades, or they may be covalently bound through hydrolyzable carbamate linkages. Hydrolysis of these carbamate linkages releases the drug at a controllable rate as the gel degrades.
1. A reactive polymer having the formula:
PEG-L-Ar—O—C(O)—X
wherein:
PEG is linear or branched poly(ethylene glycol);
L is selected from the group consisting of —O—, —S—, —NR— where R is H, C1-C6 alkyl or substituted C1-C6 alkyl, —C(O)—O—, —O—C(O)—, —O—C(O)—O—, —C(O)—NH—, —NH—C(O)—, —SO—, and —SO 2 ;
Ar is ortho-, meta-, or para-substituted phenylene; and
X is an activating group capable of reacting with a biologically active agent to form a carbamate linkage.
2. The polymer of claim 1 , wherein PEG has a molecular weight of from about 200 to 100,000 Daltons.
3. The polymer of claim 1 , wherein PEG comprises at least one capping group.
4. The polymer of claim 3 , wherein the capping group is selected from the group consisting of —OH, C1-C4 alkoxy, and -L′-Ar′—O—C(O)—X′ wherein L′, Ar′, and X′ are the same as L, Ar and X, respectively.
5. The polymer of claim 4 , wherein the capping group is methoxy.
6. The polymer of claim 1 , wherein PEG has the structure Cap-(CH 2 CH 2 O) n —CH 2 CH 2 —, wherein n is about 8 to about 4,000 and Cap is a capping group selected from the group consisting of —OH, C1-C4 alkoxy, and -L′-Ar′—O—C(O)—X′ wherein L′, Ar′, and X′ are the same as L, Ar and X, respectively.
7. The polymer of claim 1 , wherein Ar is
8. The polymer of claim 1 , wherein Ar is
9. The polymer of claim 1 , wherein X is selected from the group consisting of halogen, N-succinimidyloxy, 1-benzotriazolyloxy, and p-nitrophenyloxy.
10. The polymer of claim 9 , wherein X is Cl or Br.
11. The polymer of claim 9 , wherein X is N-succinimidyloxy.
12. The polymer of claim 9 , wherein X is 1-benzotriazolyloxy.
13. The polymer of claim 9 , wherein X is p-nitrophenyloxy.
14. The polymer of claim 1 , having the structure:
wherein mPEG is methoxy end-capped poly(ethylene glycol).
15. The polymer of claim 14 , wherein X is selected from the group consisting of halogen, N-succinimidyloxy, 1-benzotriazolyloxy, and p-nitrophenyloxy.
16. The polymer of claim 15 , wherein X is Cl or Br.
17. The polymer of claim 15 , wherein X is N-succinimidyloxy.
18. The polymer of claim 15 , wherein X is 1-benzotriazolyloxy.
19. The polymer of claim 15 , wherein X is p-nitrophenyloxy.
20. The polymer of claim 1 , having the structure:
wherein mPEG is methoxy end-capped poly(ethylene glycol).
21. The polymer of claim 20 , wherein X is selected from the group consisting of halogen, N-succinimidyloxy, 1-benzotriazolyloxy, and p-nitrophenyloxy.
22. The polymer of claim 21 , wherein X is Cl or Br.
23. The polymer of claim 21 , wherein X is N-succinimidyloxy.
24. The polymer of claim 21 , wherein X is 1-benzotriazolyloxy.
25. The polymer of claim 21 , wherein X is p-nitrophenyloxy.
26. A prodrug conjugate, comprising a polymer of claim 1 covalently attached via a carbamate linkage to a biologically active agent.
27. The conjugate of claim 26 , wherein the biologically active agent is water insoluble.
28. The conjugate of claim 26 , wherein the biologically active agent comprises at least one amino group for reaction with the polymer, the biologically active agent being selected from the group consisting of enzymes, proteins, peptides, lipids, polysaccharides, and oligonucleotides.
29. The conjugate of claim 26 , having the structure:
wherein mPEG is methoxy end-capped poly(ethylene glycol) and —NH—Y is a residue of a biologically active agent.
30. The conjugate of claim 29 , wherein the biologically active agent is water insoluble.
31. The conjugate of claim 29 , wherein the biologically active agent comprises at least one amino group for reaction with a polymer, the biologically active agent being selected from the group consisting of enzymes, proteins, peptides, lipids, polysaccharides, and oligonucleotides.
32. The conjugate of claim 26 , having the structure:
wherein mPEG is methoxy end-capped poly(ethylene glyco) and —NH—Y is a residue of a biologically active agent.
33. The conjugate of claim 32 , wherein the biologically active agent is water insoluble.
34. The conjugate of claim 32 , wherein tho biologically active agent comprises at least one amino group for reaction with a polymer, the biologically active agent being selected from the group consisting of enzymes, proteins, peptides, lipids, polysaccharides, and oligonucleotides.
35. A hydrogel comprising the polymer of claim 1 .
36. A method for preparing a prodrug conjugate, comprising:
providing a reactive polymer of claim 1 ;
reacting the polymer with Y—NH 2 , wherein Y—NH 2 is a biologically active agent comprising at least one amino group, under conditions effective to displace X and form a prodrug comprising a carbamate linkage between the biologically active agent and the polymer.
37. The method of claim 36 , wherein the prodrug has the structure:
PEG-L-Ar—O—C(O)—NH—Y
wherein —NH—Y is a residue of a biologically active agent.
38. The method of claim 37 , wherein the prodrug has the structure:
wherein mPEG is methoxy end-capped poly(ethylene glycol).
39. The method of claim 37 , wherein the prodrug has the structure:
wherein mPEG is methoxy end-capped poly(ethylene glycol).
40. The method of claim 37 , wherein the biologically active agent is water insoluble.
41. The method of claim 37 , wherein tho biologically active agent comprises at least one amino group for reaction with a polymer, the biologically active agent being selected from the group consisting of enzymes, proteins, peptides, lipids, polysaccharides, and oligonucleotides.
42. The conjugate of claim 26 , wherein the biologically active agent comprises at least one amino group for reaction with the polymer, the biologically active agent being selected from the group consisting of antibiotics, fungicides, anti-viral agents, anti-inflammatory agents, anti-tumor agents, cardiovascular agents, anti-anxiety agents, hormones, growth factors, and steroidal agents.
43. The conjugate of claim 29 , wherein the biologically active agent comprises at least one amino group for reaction with the polymer, the biologically active agent being selected from the group consisting of antibiotics, fungicides, anti-viral agents, anti-inflammatory agents, anti-tumor agents, cardiovascular agents, anti-anxiety agents, hormones, growth factors, and steroidal agents.
44. The conjugate of claim 32 , wherein the biologically active agent comprises at least one amino group for reaction with the polymer, the biologically active agent being selected from the group consisting of antibiotics, fungicides, anti-viral agents, anti-inflammatory agents, anti-tumor agents, cardiovascular agents, anti-anxiety agents, hormones, growth factors, and steroidal agents.
45. The method of claim 36 , wherein the biologically active agent comprises at least one amino group for reaction with the polymer, the biologically active agent being selected from the group consisting of antibiotics, fungicides, anti-viral agents, anti-inflammatory agents, anti-tumor agents, cardiovascular agents, anti-anxiety agents, hormones, growth factors, and steroidal agents.
46. The method of claim 36 , wherein the biologically active agent comprises at least one amino group for reaction with a polymer, the biologically active agent being selected from the group consisting of enzymes, proteins, peptides, lipids, polysaccharides, and oligonucleotides.
47. The method of claim 37 , wherein the biologically active agent comprises at least one amino group for reaction with the polymer, the biologically active agent being selected from the group consisting of antibiotics, fungicides, anti-viral agents, anti-inflammatory agents, anti-tumor agents, cardiovascular agents, anti-anxiety agents, hormones, growth factors, and steroidal agents.
48. The method of claim 36 , further comprising administering said product to a subject in need thereof.