Compounds and compositions derived from olives and methods of the use thereof
View Patent ↗The preparation of extracts from olive fruits, olive tree leaves, olive oil as well as olive-press waste. The isolation of natural products from these extracts and the evaluation of the DNA protective antioxidant activity of the extracts and the purified compounds on intact cells.
1. A composition for cytoprotection comprising a cytoprotective amount of an isolated, purified compound having the structure:
where R may be selected from the group consisting of H and C 1 -C 6 substituted and unsubstituted alkyls.
2. A composition for cytoprotection according to claim 1 , wherein R is H.
3. A composition according to claim 1 , wherein said compound is extracted from a source selected from the group of olive tree leaves, olive fruit, olive press-waste and olive oil.
4. A composition according to claim 1 , wherein said composition is provided in a dosage form selected from the group consisting of tablets, capsules, oils, creams, ointments, solutions, suspensions, shampoos, foams, hydrogels, gels, epidermal systems, transdermal systems, transmucosal systems, and parenteral systems.
5. A composition according to claim 1 , further comprising at least one antioxidant.
6. A composition according to claim 5 , wherein the antioxidant is hydroxytyrosol or tyrosol.
7. A composition according to claim 4 , wherein said composition is provided in a dosage form selected from the group consisting of buccal systems, intravenous systems, intra-muscular systems, and subcutaneous systems.
8. A composition according to claim 1 , wherein said composition is provided in a dosage form suitable for administration in a manner selected from the group consisting of oral, intra-oral, epidermal, transdermal, transmucosal, and parenteral.
9. A composition according to claim 8 , wherein said composition is provided in a dosage form suitable for administration in a manner selected from the group consisting of intravenous, intra-muscular, and subcutaneous.
10. A composition according to claim 1 , wherein said composition is at least one of a pharmaceutical composition and a cosmetic composition.
11. A composition for cytoprotection having a therapeutic amount of an isolated, purified compound having the following peaks when subjected to NMR: 1 H(400 MHz; MeOD) δ 2.51 (1H, dd, J=9.5; 16.5 Hz, H-1′a*), 2.64 (1H, dd, J=4.15; 16.45 Hz, H-1′b*), 5.64 (1H, q, J=6.5 Hz, H-1″), 1.74 (1H, d, J=7.0 Hz, H-2″), 2.71 (1H, dd, J=6.7; 15.8 Hz, H-3a*), 2.82 (1H, dd, J=6.4; 15.8 Hz, H-3b*), 3.6 (1H, m, H-4), 4.71 (2H, dd, J=12.9; 66.0 Hz, H-6); 13 C (60 MHz; MeOD) δ 35.1 (C-1′a*), 35.1 (C-1′b*), 125.6 (C-1″), 175.8 (C-2), 172.1 (C-2′), 14.2 (C-2″), 38.1 (C-3a*), 38.1 (C-3b*), 30.4 (C-4), 132.1 (C-5), 72.1 (C-6).
12. A method for protecting the DNA of living cells from damage caused by reactive oxidative species, the method comprising administering the composition according to claim 2 .
13. A method for protecting cells from damage due to oxidative stress comprises contacting said cells with a compound having the following structure:
where R may be selected from the group consisting of H and C 1 -C 6 substituted and unsubstituted alkyls.
14. The method for protecting cells according to claim 13 , wherein said compound is in a dosage form selected from the group consisting of tablets, capsules, oils, creams, ointments, solutions, suspensions, shampoos, foams, hydrogels, gels, epidermal systems, transdermal systems, transmucosal systems, and parenteral systems.
15. The method for protecting cells according to claim 13 , wherein said compound is administered by a route or system selected from the group consisting of oral, intra-oral, epidermal, transdermal, transmucosal, and parenteral.
16. The method according to claim 14 , wherein said compound is in a dosage form selected from the group consisting of buccal systems, intravenous systems, intra-muscular systems, and subcutaneous systems.
17. The method according to claim 15 , wherein said compound is administered by a route or system selected from the group consisting of intravenous, intra-muscular, and subcutaneous.
18. A method according to claim 13 , wherein said compound is in the form of at least one of a pharmaceutical composition and a cosmetic composition.
19. A method of identifying a compound as cytoprotective comprising contacting Jurkat cells with said compound, exposing said cells to a reactive oxidative species for a time period, and measuring the extent of DNA damage sustained using the comet assay.