Detection of antibodies against
View Patent ↗A new recombinant form of the plasmid-encoded protein pgp3 from C. trachomatis , serotype D, was purified by ion exchange column chromatography and shown to be suitable for quantitative immunoassy on clinical samples in an ELISA format.
1. An isolated and purified recombinant Chlamydia trachomaus pgp3 protein having its native amino acid conformation and in a form suitable for use in a serological irnmunoassay, wherein the recombinant Chlamydia trachomatis pgp3 protein is recognized by antibodies against pgp3 conformational epitopes.
2. A method of producing a recombinant Chlamydia trachomatis pgp3 protein comprising:
expressing the recombinant Chlamydia trachomatis pgp3 protein of claim 1 in a host cell; and
isolating and recovering the expressed recombinant Chlamydia trachomatis pgp3 protein under non-denaturing conditions.
3. The method of claim 2 wherein the host cell is an E. coli host cell.
4. The method of claim 2 wherein said isolating includes preparing a periplasmic extract.
5. The method of any one of claims 2 – 4 wherein said isolating includes purifying the recombinant Chlamydia trachomatis pgp3 protein using ion-exchange chromatography.