Methods of identifying individuals for inclusion in drug studies
View Patent ↗The present invention relates to genomic maps comprising biallelic markers, new biallelic markers, and methods of using biallelic markers.
1. A method of selecting an individual for inclusion in clinical trial of a drug for the treatment of Alzheimer's disease, prostate cancer or asthma comprising:
a) obtaining a nucleic acid sample from an individual;
b) constructing a nucleic acid library comprising a plurality of genomic DNA fragments that comprise the full genome of the individual or a portion of the genome of the individual;
c) determining the order of the plurality of genomic DNA fragments in the genome of the individual;
d) determining the sequence of selected regions of said plurality of genomic DNA fragments;
e) identifying nucleotides in said plurality of genomic DNA fragments that vary between individuals;
f) determining the frequencies of each allele of one or more biallelic markers in individuals showing a detectable trait;
g) identifying one or more alleles of said one or more biallelic markers that are statistically associated with said detectable trait, wherein said detectable trait is susceptibility to Alzheimer's disease and said biallelic markers are selected from: 99-365/344; the combination of biallelic markers 99-366/274, 99/344/439, 99-359/308 and 99-355/219 (IIAP8); or the combination of biallelic markers 99/359/308, 99-366/274, and 99-355/219 (IIAP7); wherein said detectable trait is susceptibility to prostate cancer and said biallelic markers are selected from the combination of 4-67 and 99-213 (haplotype 1.2); the combination of 99-217 and 4-67 (haplotype 1.1); 99-217, 4-67 and 99-213 (haplotype 2); the combination of biallelic markers 4-77, 99-217, and 4-67 (haplotype 2.2); the combination of biallelic markers 4-77, 99-217, 4-67 and 99-213 (haplotype 3.2); the combination of 99-217, 4-67, 99-213, and 99-221 (haplotype 3.1); 4-77, 99-217, 4-67, 99-213, and 99-221 (haplotype 4); the combination of 4-14, 4-77, 99-217, 4-67, 99-213, and 99-221 (haplotype 5); 4-26, 4-14, 4-77, 99-217, 4-67, 99-213, and 99-221 (haplotype 6); the combination of 4-26, 4-14, 4-77, 99-217, 4-67, 99-213, 99-221 and 99-135 (haplotype 7); or the combination of 4-26, 4-14, 4-77, 99-217, 4-67, 99-213, 99-221 and 99-135 (haplotype 8); or said detectable trait is susceptibility to asthma and said biallelic markers are selected from 32/357; 33/234; 33/327; 35/358; 35/390; the combination of biallelic markers 32/357 and 35/390 (haplotype 1); the combination of biallelic markers 33/234, 33/327, and 35/358 (haplotype 2); or the combination of biallelic markers 32/357, 33/234, 33/327, 35/358 and 35/390 (haplotype 3); and
h) including tho individual said in clinical trial if said nucleic acid sample contains one or more of said biallelic markers or combinations or biallelic markers that are associated with the detectable trait.
2. The method according to claim 1 , wherein said detectable trait is susceptibility to Alzheimer's disease and said biallelic marker is 99-365/344.
3. The method according to claim 1 , wherein said detectable trait is susceptibility to Alzheimer's disease and said biallelic markers are the combination of biallelic markers 99-366/274, 99/344/439, 99-359/308 and 99-355/219 (IIAP8).
4. The method according to claim 1 , wherein said detectable trait is susceptibility to prostate cancer and said biallelic markers are selected from the combination of 4-67 and 99-213 (haplotype 1.2).
5. The method according to claim 1 , wherein said detectable trait is susceptibility to prostate cancer and said biallelic markers are selected from the combination of 99-217 and 4-67 (haplotype 1.1).
6. The method according to claim 1 , wherein said detectable trait is susceptibility to prostate cancer and said biallelic markers are the combination of biallelic markers 99-217, 4-67 and 99-213 (haplotype 2).
7. The method according to claim 1 , wherein said detectable trait is susceptibility to prostate cancer and said biallelic markers are the combination of biallelic markers 4-77, 99-217, and 4-67 (haplotype 2.2).
8. The method according to claim 1 , wherein said detectable trait is susceptibility to prostate cancer and said biallelic markers are the combination of biallelic markers 4-77, 99-217, 4-67 and 99-213 (haplotype 3.2).
9. The method according to claim 1 , wherein said detectable trait is susceptibility to prostate cancer and said biallelic markers are the combination of biallelic markers 99-217, 4-67, 99-213, and 99-221 (haplotype 3.1).
10. The method according to claim 1 , wherein said detectable trait is susceptibility to prostate cancer and said biallelic markers are the combination of biallelic markers 4-77, 99-217, 4-67, 99-213, and 99-221 (haplotype 4).
11. The method according to claim 1 , wherein said detectable trait is susceptibility to prostate cancer and said biallelic markers are the combination of biallelic markers 4-14, 4-77, 99-217, 4-67, 99-213, and 99-221 (haplotype 5).
12. The method according to claim 1 , wherein said detectable trait is susceptibility to prostate cancer and said biallelic markers are the combination of biallelic markers 4-26, 4-14, 4-77, 99-217, 4-67, 99-213, and 99-221 (haplotype 6).
13. The method according to claim 1 , wherein said detectable trait is susceptibility to prostate cancer and said biallelic markers are the combination of biallelic markers 4-26, 4-14, 4-77, 99-217, 4-67, 99-213, 99-221 and 99-135 (haplotype 7).
14. The method according to claim 1 , wherein said detectable trait is susceptibility to prostate cancer and said biallelic markers are the combination of biallelic markers 4-26, 4-14, 4-77, 99-217, 4-67, 99-213, 99-221 and 99-135 (haplotype 8).
15. The method according to claim 1 , wherein said detectable trait is susceptibility to asthma and said biallelic marker is 32/357.
16. The method according to claim 1 , wherein said detectable trait is susceptibility to asthma and said biallelic marker is 33/234.
17. The method according to claim 1 , wherein said detectable trait is susceptibility to asthma and said biallelic marker is 33/327.
18. The method according to claim 1 , wherein said detectable trait is susceptibility to asthma and said biallelic marker is 35/358.
19. The method according to claim 1 , wherein said detectable trait is susceptibility to asthma and said biallelic marker is 35/390.
20. The method according to claim 1 , wherein said detectable trait is susceptibility to asthma and said biallelic markers are the combination of biallelic markers 32/357 and 3 5/390 (haplotype 1).
21. The method according to claim 1 , wherein said detectable trait is susceptibility to asthma and said biallelic markers are the combination of biallelic markers 33/234, 33/327, and 35/358 (haplotype 2).
22. The method according to claim 1 , wherein said detectable trait is susceptibility to asthma and said biallelic markers are the combination of biallelic markers 32/357, 33/234, 33/327, 35/358 and 35/390 (haplotype 3).