Capillary electrophoresis gel especially for separation made for single stranded nucleic acid separations
View Patent ↗A separation matrix for capillary electrophoresis of single-stranded nucleic acids is disclosed. The matrix comprises an aqueous buffer containing a denaturant and (hydroxypropyl)methyl cellulose.
1. A separation matrix for capillary electrophoresis of single-stranded nucleic acids using capillaries, the matrix comprising:
(hydroxypropyl) methyl cellulose;
an aqueous buffer solution of 20 mM tris(hydroxymethyl) aminomethane, 20 mM N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine and 0.1 mM ethylenediaminetetraacetic acid disodium salt dihydrate; and
a denaturant.
2. The separation matrix of claim 1 , wherein the denaturant is urea.
3. The separation matrix of claim 2 wherein the urea is dissolved in the matrix at a concentration of 1 M to 11 M.
4. The separation matrix of claim 3 wherein the urea is dissolved in the matrix at a concentration of 8 M.
5. The separation matrix of claim 1 wherein the (hydroxypropyl)methyl cellulose comprises 1% to 6% w/v of the matrix.
6. The separation matrix of claim 1 wherein low molecular weight (hydroxypropyl)methyl cellulose comprises 2% w/v of the matrix and higher molecular weight (hydroxypropyl)methyl cellulose comprises 1.2% w/v of the matrix.
7. A method for making a separation matrix for capillary electrophoresis comprising:
providing an aqueous buffer solution of 20 mM tris(hydroxymethyl) aminomethane, 20 mM N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine and 0.1 mM ethylenediaminetetraacetic acid disodium salt dihydrate;
providing (hydroxypropyl)methyl cellulose;
providing a denaturant; and
admixing the aqueous buffer solution, denaturant and the (hydroxypropyl)methyl cellulose.
8. The method of claim 7 wherein the denaturant is urea.
9. The method of claim 8 wherein the urea is dissolved in the matrix at a concentration of 1 M to 11 M.
10. The method of claim 9 wherein the urea is dissolved in the matrix at a concentration of 8 M.
11. The method of claim 7 wherein the (hydroxypropyl)methyl cellulose comprises 1% to 6% w/v of the matrix.
12. The method of claim 7 wherein low molecular weight (hydroxypropyl)methyl cellulose comprises 2% w/v of the matrix and higher moldcular weight (hydroxypropyl)methyl comprises 1.2% w/v of the matrix.