Nucleotide sequence which encodes a flavin monooxygenase, the corresponding protein and their uses in the spheres of diagnosis and therapy
View Patent ↗The nucleotide and polypeptide sequences of two novel human flavin-containing monooxygenase (FMO) enzymes are presented: hFMO2 and hFMOx. Vectors and host cells for cloning and/or expression of hFMO2 and hFMOx are described, as are methods of preparing hFMO2 and hFMOx polypeptides. Also included are methods for selecting compounds, diagnosing predisposition to pathologies and/or deficiencies related to FMO's, and pharmaceutical compositions containing compounds for treating and/or preventing these pathologies.
1. An isolated, purified, or recombinant polynucleotide:
a) encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 3, wherein said polypeptide has flavin monooxygenase activity;
b) encoding a polypeptide comprising SEQ ID NO: 3;
c) encoding a polypeptide comprising a fragment of SEQ ID NO: 3, wherein said polypeptide and said fragment has flavin monooxygenase activity;
d) encoding a polypeptide consisting of a fragment of SEQ ID NO: 3; or
e) comprising a polynucleotide sequence completely complementary to a polynucleotide as set forth in a), b), c), or d).
2. The isolated, purified, or recombinant polynucleotide according to claim 1 , wherein the polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 3, wherein said polypeptide has flavin monooxygenase activity.
3. The isolated, purified, or recombinant polynucleotide according to claim 1 , wherein the polynucleotide encodes a polypeptide comprising a fragment of SEQ ID NO: 3, wherein said polypeptide and said fragment has flavin monooxygenase activity.
4. The isolated, purified, or recombinant polynucleotide according to claim 1 , wherein said polynucleotide encodes a polypeptide comprising SEQ ID NO: 3.
5. The isolated, purified, or recombinant polynucleotide according to claim 1 , wherein said polynucleotide encodes a polypeptide consisting of a fragment of SEQ ID NO: 3.
6. The isolated, purified, or recombinant polynucleotide according to claim 1 , wherein the polynucleotide comprises a polynucleotide sequence completely complementary to an isolated, purified, or recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 3, wherein said polypeptide has flavin monooxygenase activity.
7. The isolated, purified, or recombinant polynucleotide according to claim 1 , wherein the polynucleotide comprises a polynucleotide sequence completely complementary to an isolated, purified, or recombinant polynucleotide encoding a polypeptide comprising SEQ ID NO: 3.
8. The isolated, purified, or recombinant polynucleotide according to claim 1 , wherein the polynucleotide comprises a polynucleotide sequence completely complementary to an isolated, purified, or recombinant polynucleotide encoding a polypeptide comprising a fragment of SEQ ID NO: 3, wherein said polypeptide and said fragment has flavin monooxygenase activity.
9. The isolated, purified, or recombinant polynucleotide according to claim 1 , wherein the polynucleotide comprises a polynucleotide sequence completely complementary to an isolated, purified, or recombinant polynucleotide encoding a polypeptide consisting of a fragment of SEQ ID NO: 3.
10. A cloning or expression vector comprising an isolated, purified, or recombinant polynucleotide:
a) encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 3, wherein said polypeptide has flavin monooxygenase activity;
b) encoding a polypeptide comprising SEQ ID NO: 3;
c) encoding a polypeptide comprising a fragment of SEQ ID NO: 3, wherein said polypeptide and said fragment has flavin monooxygenase activity;
d) encoding a polypeptide consisting of a fragment of SEQ ID NO: 3; or
e) comprising a polynucleotide sequence completely complementary to a polynucleotide as set forth in a), b), c), or d).
11. The cloning or expression vector according to claim 10 , wherein the vector is an expression vector.
12. The cloning or expression vector according to claim 10 , wherein the vector is a cloning vector.
13. The cloning or expression vector according to claim 10 , wherein the vector further comprises promoter sequences and regulatory sequences.
14. The cloning or expression vector according to claim 11 , wherein the vector further comprises promoter sequences and regulatory sequences.
15. The cloning or expression vector according to claim 12 , wherein the vector further comprises promoter sequences and regulatory sequences.
16. The cloning or expression vector according to claim 10 , wherein the vector comprises a polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 3, wherein said polypeptide has flavin monooxygenase activity.
17. The cloning or expression vector according to claim 10 , wherein the vector comprises a polynucleotide encoding a polypeptide comprising a fragment of SEQ ID NO: 3, wherein said polypeptide and said fragment has flavin monooxygenase activity.
18. The cloning or expression vector according to claim 10 , wherein the vector comprises a polynucleotide encoding a polypeptide comprising SEQ ID NO: 3.
19. The cloning or expression vector according to claim 10 , wherein the vector comprises a polynucleotide encoding a polypeptide consisting of a fragment of SEQ ID NO: 3.
20. The cloning or expression vector according to claim 10 , wherein the vector comprises a polynucleotide comprising a polynucleotide sequence completely complementary to an isolated, purified, or recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 3, wherein said polypeptide has flavin monooxygenase activity.
21. The cloning or expression vector according to claim 10 , wherein the vector comprises a polynucleotide comprising a polynucleotide sequence completely complementary to an isolated, purified, or recombinant polynucleotide encoding a polypeptide comprising SEQ ID NO: 3.
22. The cloning or expression vector according to claim 10 , wherein the vector comprises a polynucleotide comprising a polynucleotide sequence completely complementary to an isolated, purified, or recombinant polynucleotide encoding a polypeptide comprising a fragment of SEQ ID NO: 3, wherein said polypeptide and said fragment has flavin monooxygenase activity.
23. The cloning or expression vector according to claim 10 , wherein the vector comprises a polynucleotide comprising a polynucleotide sequence completely complementary to an isolated, purified, or recombinant polynucleotide encoding a polypeptide consisting of a fragment of SEQ ID NO: 3.
24. The cloning or expression vector according to claim 10 , wherein said vector comprises a polynucleotide sequence completely complementary to an isolated, purified, or recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 3, wherein said polypeptide has flavin monooxygenase activity.
25. A host cell comprising a polynucleotide or vector according to claim 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , or 24 .
26. The host cell according to claim 25 , wherein said host cell comprises a polynucleotide according to claim 2 .
27. The host cell according to claim 25 , wherein said host cell comprises a polynucleotide according to claim 3 .
28. The host cell according to claim 25 , wherein said host cell comprises a polynucleotide according to claim 4 .
29. The host cell according to claim 25 , wherein said host cell comprises a polynucleotide according to claim 5 .
30. The host cell according to claim 25 , wherein said host cell comprises a polynucleotide according to claim 6 .
31. The host cell according to claim 25 , wherein said host cell comprises a polynucleotide according to claim 7 .
32. The host cell according to claim 25 , wherein said host cell comprises a polynucleotide according to claim 8 .
33. The host cell according to claim 25 , wherein said host cell comprises a polynucleotide according to claim 9 .
34. The host cell according to claim 25 , wherein said host cell comprises a vector according to claim 11 .
35. The host cell according to claim 25 , wherein said host cell comprises a vector according to claim 12 .
36. The host cell according to claim 25 , wherein said host cell comprises a vector according to claim 13 .
37. The host cell according to claim 25 , wherein said host cell comprises a vector according to claim 14 .
38. The host cell according to claim 25 , wherein said host cell comprises a vector according to claim 15 .
39. The host cell according to claim 25 , wherein said host cell comprises a vector according to claim 16 .
40. The host cell according to claim 25 , wherein said host cell comprises a vector according to claim 17 .
41. The host cell according to claim 25 , wherein said host cell comprises a vector according to claim 18 .
42. The host cell according to claim 25 , wherein said host cell comprises a vector according to claim 19 .
43. The host cell according to claim 25 , wherein said host cell comprises a vector according to claim 20 .
44. The host cell according to claim 25 , wherein said host cell comprises a vector according to claim 21 .
45. The host cell according to claim 25 , wherein said host cell comprises a vector according to claim 22 .
46. The host cell according to claim 25 , wherein said host cell comprises a vector according to claim 23 .
47. The host cell according to claim 25 , wherein said host cell comprises a vector according to claim 24 .