IP Library Granted Patent US 8,916,114
Granted Patent B2
US 8,916,114 · App. 10/393,911 · Granted Dec 23, 2014

Microtitration plate

Inventor: Martin Seippel (Oldenburg, DE)
Assignee: Eppendorf AG
B01L3/50851B01L3/5085B29C45/16B01L2200/12B01L2300/0829B29C2045/1659
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Quick Facts
Patent No.
US 8,916,114
App. No.
10/393,911
Granted
Dec 23, 2014
Kind
B2
Abstract

A microtitration plate, having a frame made of a plastic which has a plate with a multiplicity of holes, and a multiplicity of receptacles made of the same plastic which are fixedly connected to the plate at the holes by directly molding them thereto, have a receiving portion protruding from the underside of the plate, and are accessible from the upper surface of the plate through apertures.

Claims (28)

1. A microtitration plate for use for automated polymerase chain reaction (PCR), comprising:

a frame ( 2 ) which has a plate ( 4 ) wherein the plate ( 4 ) has a multiplicity of holes ( 6 ′) molded of a polypropylene; and

a multiplicity of thin-wall receptacles ( 3 ) having a wall thickness of from about 0.05 to about 0.25 mm at least in one wall portion, and made of the identical type of polypropylene as the frame ( 2 ) and the plate ( 4 ), with the receptacles ( 3 ) fixedly connected to the plate ( 4 ) at the holes ( 6 ′) by fusing to each other the identical type of polypropylene of the receptacles ( 3 ) and the plate ( 4 ) due to direct molding of the receptacles ( 3 ) to the plate ( 4 ), the receptacles ( 3 ) having receiving portions ( 9 , 10 , 11 ) protruding from the underside ( 8 ) of the plate ( 4 ), and are accessible from the upper surface ( 7 ) of the plate ( 4 ) through apertures ( 15 ), wherein the frame ( 2 ) and the receptacles ( 3 ) are composed of the identical type of polypropylene having different colors.

2. The microtitration plate according to claim 1 , wherein the receptacles ( 3 ) have a wall portion ( 10 ) of a very small wall thickness adjacent to a receptacle bottom ( 9 ) and have an upper wall portion ( 12 ) connected to the plate ( 4 ).

3. The microtitration plate according to claim 1 , wherein the receptacles ( 3 ) have a collar of an increased wall thickness as an upper wall portion ( 12 ) connected to the plate ( 4 ).

4. The microtitration plate according to claim 1 , wherein each receptacle ( 3 ) has

a substantially cup-shaped bottom ( 9 ),

a wall portion ( 10 ) of small wall thickness that is substantially conical, and

a wall portion ( 11 ) of a wall thickness which gradually increases upwardly adjoining the wall portion ( 10 ) of a small wall thickness and an upper wall portion ( 12 ) connected to the plate ( 4 ) and extending to an opposing side of the plate ( 4 ).

5. The microtitration plate according to claim 1 , wherein the receptacles ( 3 ) have molding points at the bottom ( 9 ) of the receptacles.

6. The microtitration plate according to claim 1 , wherein the frame ( 2 ) has a bordering ( 5 ) protruding from the underside ( 8 ) thereof at the edge of the plate ( 4 ).

7. The microtitration plate according to claim 1 , wherein the frame ( 2 ) has several edge-sided molding points.

8. The microtitration plate according to claim 1 , which is made of a polypropylene including a filler which does not contain protein.

9. The microtitration plate according to claim 1 , which is made of a polypropylene filled with fiberglass.

10. A microtitration plate for use for automated polymerase chain reaction (PCR), comprising:

a frame ( 2 ) which has a plate ( 4 ) wherein the plate ( 4 ) has a multiplicity of holes ( 6 ′) molded of a polycarbonate

a multiplicity of thin-wall receptacles ( 3 ) having a wall thickness of from about 0.05 to about 0.25 mm at least in one wall portion, and molded of a polycarbonate, with the receptacles ( 3 ) fixedly connected to the plate ( 4 ) at the holes ( 6 ′) by fusing to each other both the polycarbonate of the plate ( 4 ) and the polycarbonate of the receptacles ( 3 ) due to direct molding of the receptacles ( 3 ) to the plate ( 4 ), the receptacles ( 3 ) having receiving portions ( 9 , 10 , 11 ) protruding from the underside ( 8 ) of the plate ( 4 ), and are accessible from the upper surface ( 7 ) of the plate ( 4 ) through apertures ( 15 ), wherein the frame ( 2 ) and the receptacles ( 3 ) are composed of polycarbonates having different colors.

11. The microtitration plate according to claim 10 , wherein the receptacles ( 3 ) have a wall portion ( 10 ) of a very small wall thickness adjacent to a receptacle bottom ( 9 ) and have an upper wall portion ( 12 ) connected to the plate ( 4 ).

12. The microtitration plate according to claim 10 , wherein the receptacles ( 3 ) have a collar of an increased wall thickness as an upper wall portion ( 12 ) connected to the plate ( 4 ).

13. The microtitration plate according to claim 10 , wherein each receptacle ( 3 ) has

a substantially cup-shaped bottom ( 9 ),

a wall portion ( 10 ) of small wall thickness that is substantially conical, and

a wall portion ( 11 ) of a wall thickness which gradually increases upwardly adjoining the wall portion ( 10 ) of a small wall thickness and an upper wall portion ( 12 ) connected to the plate ( 4 ) and extending to an opposing side of the plate ( 4 ).

14. The microtitration plate according to claim 10 , wherein the receptacles ( 3 ) have molding points at the bottom ( 9 ) of the receptacles.

15. The microtitration plate according to claim 10 , wherein the frame ( 2 ) has a bordering ( 5 ) protruding from the underside ( 8 ) thereof at the edge of the plate ( 4 ).

16. The microtitration plate according to claim 10 , wherein the frame ( 2 ) has several edge-sided molding points.

17. The microtitration plate according to claim 10 , which is made of a temperature-resistant polycarbonate.

18. The microtitration plate according to claim 10 , which is made of a chemical-resistant polycarbonate.

Assignments (2)
CHANGE OF NAME Recorded Mar 7, 2023
From: EPPENDORF AG
To: EPPENDORF SE
Reel/Frame 062967/0806 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 21, 2003
From: SEIPPEL, MARTIN
To: EPPENDORF AG
Reel/Frame 013906/0863 →
Priority Claims (1)
DE 102 12 761 · Mar 22, 2002 · national
Continuity (1)
Related Publication 20030180192A1 · Sep 25, 2003