Transformation system in the field of filamentous fungal hosts
View Patent ↗A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium , more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.
1. A mutant Chrysosporium strain comprising a nucleic acid sequence encoding a polypeptide of interest, said nucleic acid sequence being operably linked to a promoter sequence and optionally a secretion signal sequence, said mutant strain expressing said polypeptide of interest at a higher level than the corresponding non-mutant strain under the same conditions, wherein said mutant derived from Chrysosporium lucknowense is UV13-6 (VKM F-3632 D), NG7C-19 (VKM F-3633 D), or UV18-25 (VKM F-3631 D) or is derived from a Chrysosporium lucknowense strain of C. lucknowense strain C1 (VKM F-3500 D), UV13-6 (VKM F-3632 D), NG7C-19 (VKM F-3633 D), or UV18-25 (VKM F-3631 D).
2. A mutant Chrysosporium strain wherein said mutant is or is derived from a Chrysosporium lucknowense mutant strain selected from the group consisting of UV13-6 (VKM F-3632 D), NG7C-19 (VKM F-3633 D), and UV18-25 (VKM F-3631 D).
3. The mutant Chrysosporium strain of claim 2 wherein said mutant is or is derived from a Chrysosporium lucknowense mutant strain UV13-6 (VKM F-3632 D).
4. The mutant Chrysosporium strain of claim 2 wherein said mutant is or is derived from a Chrysosporium lucknowense mutant strain NG7C-19 (VKM F-3633 D).
5. The mutant Chrysosporium strain of claim 2 wherein said mutant is or is derived from a Chrysosporium lucknowense mutant strain UV18-25 (VKM F-3631 D).
6. The mutant Chrysosporium strain of claim 2 , 3 , 4 or 5 wherein said mutant strain expresses a polypeptide of interest at a higher level than the corresponding original mutant strain under the same conditions.