Generation of plants with improved pathogen resistance
View Patent ↗The present invention is directed to plants that display a pathogen resistance phenotype due to altered expression of a PPR1 nucleic acid. The invention is further directed to methods of generating plants with a pathogen resistance phenotype.
1. A method of producing increased pathogen resistance in a plant, said method comprising:
a) introducing into progenitor cells of the plant a plant transformation vector comprising a nucleotide sequence encoding SEQ ID NO: 3, or a polypeptide having at least 95% sequence identity with SEQ ID NO: 3, wherein the polypeptide confers Peronspora parasitica resistance (PPR1) activity; and
b) growing the transformed progenitor cells to produce a transgenic plant, wherein said transgenic plant exhibits constitutive expression of endogenous PR1 protein and increased resistance to a pathogen controlled by the salicylic acid-dependent resistance pathway relative to a control plant.
2. A transformed plant produced by the method of claim 1 .
3. A transformed plant part obtained from the plant according to claim 2 .
4. A transgenic plant comprising a plant transformation vector comprising a nucleotide sequence encoding a PPR1 polypeptide having at least 95% sequence identity with SEQ ID NO:3, wherein said transgenic plant exhibits constitutive expression of endogenous PR1 protein and has increased resistance to a pathogen controlled by salicylic acid-dependent resistance pathway relative to a control plant.
5. The transgenic plant of claim 4 , wherein the PPR1 polypeptide is SEQ ID NO:3.