Micromanifold assembly
View Patent ↗A micromanifold for connecting external capillaries to the inlet and/or outlet ports of a microfluidic device can employ a ferrule/capillary assembly that includes: (a) a ferrule comprising an elongated member and having a bore traversing from a proximal end to a distal end of the member, wherein the bore has an inner surface and wherein the distal end of the ferrule has a tapered, threaded exterior surface, and (b) a capillary that is positioned within the bore wherein the capillary's outer surface is in direct contact with the bore's inner surface. No mating sleeve is required for the one-piece ferrule. Alternatively, the capillaries can be bonded to channels that traverse the manifold and therefore obviate the need for a ferrule.
1. A fluid connector assembly coupling at least one fluid source to at least one port of a microfluidic device comprising:
a manifold comprising one or more channels extending therethrough, and a bottom surface that defines one or more channel outlets, wherein the one or more channels each comprise an inner surface configuration having a threaded upper portion and a tapered lower portion, and wherein the bottom surface further comprises an annular groove surrounding each of the one or more channel outlets, wherein each of the annular grooves includes an o-ring seal;
a microfluidic device having one or more ports on a top surface, the top surface positioned against the bottom surface of the manifold, wherein each of the one or more ports is aligned with a corresponding one of the one or more channel outlets and wherein the so aligned port(s) and channel(s) are in fluid communication;
one or more elongated fluid conduits;
a fluid source;
means for securing a proximal end of each of the one or more elongated fluid conduits to each of the one or more channels, wherein a distal end of at least one of the one or more elongated fluid conduits is connected to the fluid source, the securing means comprising a ferrule, comprising a threaded middle portion, a tapered lower end, and an axial bore extending therethrough into which one of the one or more elongated fluid conduits is disposed, wherein the axial bore adjacent to the tapered lower end of the ferrule is compressed against the elongated fluid conduit disposed therein as the ferrule is inserted and screwed into the threaded upper portion of one of the one or more channels and is tightened thereby securing the proximal end of the elongated fluid conduit; and
a compression plate disposed adjacent to a bottom surface of the microfluidic device, and a plurality of fasteners securing the microfluidic device between compression plate and the manifold with sufficient force that the o-ring seals form a fluid tight seal between the bottom surface of the manifold and the top surface of the microfluidic device.
2. The assembly of claim 1 wherein at least one of the one or more elongated fluid conduits is a capillary.
3. The assembly of claim 1 wherein the ferrule does not include a mating sleeve around the elongated fluid conduit disposed within the ferrule so that an outer surface of the elongated fluid conduit is in direct contact with the surface of the axial bore.
4. The assembly of claim 1 wherein the plurality of fasteners are selected from the group consisting of bolts, spring loaded bolts, screw, spring loaded screws, clamps, electrically activated solenoids, and combinations thereof.
5. The assembly of claim 1 wherein the microfluidic device is held in position between the manifold and the compression plate by a power generated device that generates a compressive force.
6. The assembly of claim 1 wherein at least one of the one or more elongated fluid conduits is a capillary having an inner diameter that ranges from 5 microns to 250 microns.
7. The assembly of claim 1 wherein the o-ring is made of a plastic or silicone rubber.
8. The assembly of claim 1 wherein the one of the one or more elongated fluid conduits is a hollow needle and the fluid source is a reservoir having a chamber that is separated by a septum, wherein the proximal end of the hollow needle pierces the septum so that the chamber is in fluid communication with one of the one or more ports of the microfluidic device.
9. The assembly of claim 8 wherein the reservoir has an elongated nozzle having an aperture that is covered by the septum and at least part of the elongated nozzle is situated within at least one channel.